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Status |
Public on May 15, 2019 |
Title |
BO112_B16-OVA_1 |
Sample type |
RNA |
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|
Source name |
B16-OVA cell line incubated with BO-112 replicate 1
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Organism |
Mus musculus |
Characteristics |
tissue: B16-OVA cell line
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Treatment protocol |
B16-OVA cells were incubated either with BO-112 at 0.5 µg/mL (n=3) or identical volumes of vehicle (5%glucose, n=3). After 24h, cells were collected and total RNA was isolated in two steps using TRIzol (Life technologies) and Rneasy Mini-Kit (Quiagen) purification, following manufacturer`s RNA cleanup protocol.
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Growth protocol |
B12-OVA cells were cultured in RPMI 1640 containing 10% fetal bovine serum, 2mM glutamine, 100 U/ml penicillin and 100 µg/ml streptomycin, and 50 µM 2-mercaptoethanol and 400µg/mL Geneticin (Gibco).
|
Extracted molecule |
total RNA |
Extraction protocol |
The cells were harvested with TRIzol Reagent (Invitrogen) and the RNA was extracted according to the manufacturer's instructions. As a last step of the extraction procedure, the RNA was purified with the RNeasy Mini-kit (Qiagen, Hilden, Germany).
|
Label |
Biotin
|
Label protocol |
The sense cDNA was prepared from 300 ng of total RNA using the Ambion® WT Expression Kit. The sense strand cDNA was then fragmented and biotinylated with the Affymetrix GeneChip® WT Terminal Labeling Kit (PN 900671).
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Hybridization protocol |
Labeled sense cDNA was hybridized to the Affymetrix Mouse Clariom S array according to the the manufacturer protocols and using GeneChip® Hybridization, Wash and Stain Kit.
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Scan protocol |
Genechips were scanned with the Affymetrix GeneChip® Scanner 3000.
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Description |
Gene expression data from whole B16-OVA cell line
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Data processing |
Data was normalized using Bioconductor software and RMA algorithm.
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Submission date |
Jun 20, 2018 |
Last update date |
May 15, 2019 |
Contact name |
Maria Angela Aznar |
E-mail(s) |
maznargo@alumni.unav.es
|
Organization name |
Center for applied medical research (CIMA)
|
Street address |
Pio XII num 55
|
City |
Pamplona |
ZIP/Postal code |
E-31008 |
Country |
Spain |
|
|
Platform ID |
GPL23038 |
Series (2) |
GSE116075 |
Immunotherapeutic effects of intratumoral nanoplexed Poly I:C (B16-OVA cell line) |
GSE116078 |
Immunotherapeutic effects of intratumoral nanoplexed Poly I:C |
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