|
| Status |
Public on Jun 04, 2018 |
| Title |
Control 2 Beads with IL-6 stim |
| Sample type |
RNA |
| |
|
| Source name |
Control Beads with IL-6 stim
|
| Organism |
Homo sapiens |
| Characteristics |
subject status: control
tissue: blood
cell type: Naive CD4+ T cells
stimulated with: CD2/CD3/CD28-coated beads (Miltenyi) + IL-6 (100ng/mL)
|
| Treatment protocol |
Naive CD4+ T cells from patients or controls were stimulated with CD2/CD3/CD28-coated beads (Miltenyi) + IL-6 (100ng/mL). HVS-transformed Saimiri T cells were used without stimulation.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Minikit Qiagen
|
| Label |
biotin
|
| Label protocol |
25 ng of total RNA is reverse transcribed following the Ovation PicoSL V2 (Nugen). Briefly, the resulting double strand cDNA is used for SPIA amplification. 3,6 ug of amplified cDNA are fragmented and biotin labelled using Encore Biotin Module (Nugen).
|
| |
|
| Hybridization protocol |
3,6 ug of Biotynilated single stranded sense cDNA is then hybridized to GeneChip® Clariom S Human (Affymetrix-Thermofisher) at 45°C for 17 hours
|
| Scan protocol |
After overnight hybridization, chips are washed on the fluidic station FS450 following specific protocols (Affymetrix) and scanned using the GCS3000 7G
|
| Data processing |
The raw CEL files were imported and analyzed using 'affy' package in R language. Data was normalized using RMA normalization and log2 transformed.
|
| |
|
| Submission date |
May 02, 2018 |
| Last update date |
Jun 04, 2018 |
| Contact name |
Tanwir Habib |
| E-mail(s) |
thabib@sidra.org
|
| Organization name |
Sidra Medicine
|
| Street address |
Al Luqta Street
|
| City |
Doha |
| ZIP/Postal code |
26999 |
| Country |
Qatar |
| |
|
| Platform ID |
GPL23159 |
| Series (1) |
| GSE113945 |
Human ZNF341 deficiency underlies a recessive form of hyper IgE syndrome by disrupting STAT3 transcription-dependent STAT3 activity |
|
