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Sample GSM30467

Query DataSets for GSM30467

Status

Public on Sep 15, 2004

Title

G-PBMC monocyte 4

Sample type

RNA

Source name

CD14+ cells from G-CSF mobilized peripheral blood

Organism

Homo sapiens

Extracted molecule

total RNA

Description

CD14+ cells were isolated from peripheral blood mononuclear cells collected from normal donors after 3 days of G-CSF stimulation. Dako Tuek4 antibody and Rat anti-mouse IgG2a+b magnetic bead selection were used to isolate cells (>95% purity). Total RNA was isolated with Qiagen RNeasy columns and converted to biotin-labeled cRNA by standard Affymetrix protocols. Targets were produced using standard Affymetrix procedures from about 12ug of total RNA. For analysis with MAS5 the sample was scaled to an arbitrary value of 1000 by overall hybridization intensity to allow comparison of multiple samples.
Keywords = monocyte, CD14, GPBMC

Submission date

Sep 10, 2004

Last update date

May 27, 2005

Contact name

Lynn Graf

E-mail(s)

lgraf@fhcrc.org

Phone

206-667-4545

Fax

206-667-5978

URL

http://none

Organization name

Fred Hutchinson Cancer Research Center

Department

Clinical Research Division

Lab

Torok-Storb

Street address

1100 Fairview Ave. N. D1-100

City

Seattle

State/province

ZIP/Postal code

98109

Country

USA

Platform ID

GPL80

Series (1)

GSE1746

CD14+ cell analysis

Data table header descriptions
ID_REF
VALUE	'signal', a measure of the abundance of a transcript
ABS_CALL	the call in an absolute analysis that indicates if the transcript was present (P), absent (A) or marginal (M)
DETECTION P-VALUE	'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF	VALUE	ABS_CALL	DETECTION P-VALUE
A28102_at	256.2	A	0.156732
AB000114_at	67.8	A	0.262827
AB000115_at	327.4	P	0.036569
AB000220_at	323	P	0.001796
AB000381_s_at	29.6	A	0.617401
AB000409_at	1871.2	P	0.000258
AB000410_s_at	135.6	A	0.686277
AB000449_at	265.4	M	0.058444
AB000450_at	493.8	P	0.021902
AB000460_at	2020.4	P	0.004017
AB000462_at	331.7	A	0.185131
AB000464_at	915.8	P	0.003212
AB000466_at	117.3	A	0.960339
AB000467_at	211.1	A	0.753049
AB000468_at	2647.5	P	0.00034
AB000584_at	744.7	A	0.354453
AB000816_s_at	39.3	A	0.52976
AB000895_at	177	A	0.724854
AB000896_at	413.2	A	0.300606
AB000897_at	62.5	A	0.749204

Total number of rows: 7129

Table truncated, full table size 194 Kbytes.

Supplementary file	Size	Download	File type/resource
GSM30467.cel.gz	2.3 Mb	(ftp)(http)	CEL