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Sample GSM30464

Query DataSets for GSM30464

Status

Public on Sep 15, 2004

Title

G-PBMC monocyte 1

Sample type

RNA

Source name

CD14+ cells from G-CSF mobilized peripheral blood

Organism

Homo sapiens

Extracted molecule

total RNA

Description

CD14+ cells were isolated from peripheral blood mononuclear cells collected from normal donors after 3 days of G-CSF stimulation. Dako Tuek4 antibody and Rat anti-mouse IgG2a+b magnetic bead selection were used to isolate cells (>95% purity). Total RNA was isolated with Qiagen RNeasy columns and converted to biotin-labeled cRNA by standard Affymetrix protocols. Targets were produced using standard Affymetrix procedures from about 12ug of total RNA. For analysis with MAS5 the sample was scaled to an arbitrary value of 1000 by overall hybridization intensity to allow comparison of multiple samples.
Keywords = monocyte, CD14, GPBMC

Submission date

Sep 10, 2004

Last update date

May 27, 2005

Contact name

Lynn Graf

E-mail(s)

lgraf@fhcrc.org

Phone

206-667-4545

Fax

206-667-5978

URL

http://none

Organization name

Fred Hutchinson Cancer Research Center

Department

Clinical Research Division

Lab

Torok-Storb

Street address

1100 Fairview Ave. N. D1-100

City

Seattle

State/province

ZIP/Postal code

98109

Country

USA

Platform ID

GPL80

Series (1)

GSE1746

CD14+ cell analysis

Data table header descriptions
ID_REF
VALUE	'signal', a measure of the abundance of a transcript
ABS_CALL	the call in an absolute analysis that indicates if the transcript was present (P), absent (A) or marginal (M)
DETECTION P-VALUE	'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF	VALUE	ABS_CALL	DETECTION P-VALUE
A28102_at	503.9	P	0.030976
AB000114_at	94.5	A	0.108979
AB000115_at	463.8	P	0.033677
AB000220_at	387.8	P	0.000581
AB000381_s_at	85.2	A	0.287743
AB000409_at	2281.8	P	0.00007
AB000410_s_at	86.5	A	0.814869
AB000449_at	278.3	P	0.039661
AB000450_at	510.2	P	0.009337
AB000460_at	1683	P	0.00141
AB000462_at	445.8	A	0.156732
AB000464_at	529.6	P	0.006187
AB000466_at	101.3	A	0.969024
AB000467_at	227.6	A	0.897835
AB000468_at	1210.3	P	0.00039
AB000584_at	824.1	A	0.275146
AB000816_s_at	21.7	A	0.672921
AB000895_at	138.7	A	0.737173
AB000896_at	181.7	A	0.58862
AB000897_at	115	A	0.686277

Total number of rows: 7129

Table truncated, full table size 193 Kbytes.

Supplementary file	Size	Download	File type/resource
GSM30464.cel.gz	2.2 Mb	(ftp)(http)	CEL