|
| Status |
Public on Dec 23, 2019 |
| Title |
CD62L low CD4+ T cells from non-responder of patient 2; ICI-52 |
| Sample type |
RNA |
| |
|
| Source name |
CD62L low CD4+ T cells_non-responder
|
| Organism |
Homo sapiens |
| Characteristics |
subject id: ICI-52
response to nivolumab treatment: non-responder
cell: CD62L low CD4+ T cells
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated ssDNA were prepared according to the standard Affymetrix protocol from 0.5 ug total RNA (GeneChip™ WT PLUS Reagent Kit Manual, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 1.8 ug of ssDNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner Scanner 3000 7G.
|
| Description |
NR_CD62L_low_CD4+_2
|
| Data processing |
The data were analyzed with Expression Console™ Software 1.4 using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Aug 28, 2017 |
| Last update date |
Dec 24, 2019 |
| Contact name |
Masashi Kitazawa |
| E-mail(s) |
masashi-kitazawa@aist.go.jp
|
| Organization name |
National of Institute of Advanced Industrial Science and Technology (AIST)
|
| Street address |
2-4-7 Aomi
|
| City |
Koto-ku, |
| State/province |
Tokyo |
| ZIP/Postal code |
135-0064 |
| Country |
Japan |
| |
|
| Platform ID |
GPL23159 |
| Series (1) |
| GSE103157 |
Gene Expressions responsible for good response to Nivolumab treatment |
|
