|
| Status |
Public on Jan 19, 2018 |
| Title |
F7 wt HU-60' |
| Sample type |
SRA |
| |
|
| Source name |
Genomic DNA
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
strain: W303
genotype/variation: Wild type 60' after release in S-Phase in presence of HU(200mM)
|
| Treatment protocol |
Samples were released from alpha-factor arrest in the presence of 200 mM HU (hydroxyurea).
|
| Growth protocol |
Cells were grown on YPD at 25°C
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Standard protocol for preparation of yeast genomic DNA
NeoPrep Illumina system for automatic NGS library preparation using TruSeq Nano DNA Library Prep Kit for NeoPrep (Illumia)_Sequencer:HiSeq 4000 (1x51 nt)
|
| |
|
| Library strategy |
OTHER |
| Library source |
genomic |
| Library selection |
other |
| Instrument model |
Illumina HiSeq 4000 |
| |
|
| Data processing |
Basecalls performed using HiSeq Control Software 1.5.15.1, RTA 1.13.48.0 and CASAVA version 1.8.2
Reads were aligned to the sacCer3 genome assembly using using Bowtie 2, version 2.1.0, allowing only perfect matches
bigwig files were generated with the tool "Bamcompare" from Deeptool2. Ramírez et al, (2016)
Genome_build: sacCer3
Supplementary_files_format_and_content: Bigwig files correspond to signal ratio smoothed with a 1000 bp window and a 1 bp step
|
| |
|
| Submission date |
Aug 24, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
romain forey |
| E-mail(s) |
romain.forey@epfl.ch
|
| Organization name |
IGH
|
| Street address |
142 avenue de la Cardonille
|
| City |
Montpellier |
| ZIP/Postal code |
34000 |
| Country |
France |
| |
|
| Platform ID |
GPL21656 |
| Series (1) |
| GSE103073 |
Dbf4 recruitment by forkhead transcription factors defines an upstream rate-limiting step in determining replication origin firing |
|
| Relations |
| SRA |
SRX3130802 |
| BioSample |
SAMN07559518 |
