|
| Status |
Public on Feb 01, 2009 |
| Title |
card9 TDB |
| Sample type |
RNA |
| |
|
| Source name |
Macrophages
|
| Organism |
Mus musculus |
| Characteristics |
card 9 KO, TDB
|
| Treatment protocol |
cells were stimulated with 40 µg/ml TDB for 48h
|
| Growth protocol |
Macrophages were differentiated from bone marrow cells in cDMEM containing 10 % L-cell conditioned medium for 6 days and prestimulated with 10 ng/ml interferon gamma for 3h
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNeasy Mini Kit
|
| Label |
biotin, PE
|
| Label protocol |
samples were processed with the Whole Transcript Assay Kit as recommended by the manufacterer (Affymetrix)
|
| |
|
| Hybridization protocol |
Affymetrix standard procedure
|
| Scan protocol |
Affymetrix standard procedure; Affymetrix Scanner 3000 7G
|
| Description |
macrophages from wt mice, not stimulated
|
| Data processing |
rma background correction and normalization. Linear values
|
| |
|
| Submission date |
Feb 14, 2008 |
| Last update date |
Feb 19, 2008 |
| Contact name |
Joerg Mages |
| E-mail(s) |
mages@lrz.tum.de
|
| Organization name |
Technical University Munich
|
| Department |
Institute of Medical Microbiology, Immunology and Hygiene
|
| Street address |
Trogerstrasse 30
|
| City |
Munich |
| ZIP/Postal code |
81675 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE10530 |
Card9 dependent activation of macrophages by TDB |
|
