|
| Status |
Public on Dec 04, 2008 |
| Title |
Control hypothyroid CH_20 |
| Sample type |
RNA |
| |
|
| Source name |
control hypothyroid
|
| Organism |
Mus musculus |
| Characteristics |
Mouse liver total RNA; mixed strain background (B6;129S); NCoRloxP/loxP ; mice were put on a low iodine diet supplemented with 0.15% PTU (LoI/PTU-Harlan Teklad TD.95125) for 3 weeks.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from was isolated using Trizol as described above and further purified with RNeasy Mini kit per manufacturer’s instructions (Qiagen, Valencia, CA). 200 ng total RNA was then used for GeneChip analysis. Preparation of terminal-labeled cDNA, hybridization to genome-wide murine Gene Level 1.0 ST GeneChips (Affymetrix, Santa Clara, CA, USA) and scanning of the arrays were carried out according to manufacturer’s protocols (https://www.affymetrix.com).
|
| Label |
Biotin
|
| Label protocol |
WT labeling kit (www.affymetrix.com)
|
| |
|
| Hybridization protocol |
affymetrix
|
| Scan protocol |
affymetrix
|
| Description |
affymetrix
|
| Data processing |
RMA signal intensity calculation
|
| |
|
| Submission date |
Dec 21, 2007 |
| Last update date |
Dec 03, 2008 |
| Contact name |
Martin Bilban |
| E-mail(s) |
Martin.Bilban@meduniwien.ac.at
|
| Phone |
++43 (0)1 40400 6441
|
| Organization name |
Medical University of Vienna
|
| Department |
|
| Street address |
|
| City |
Vienna |
| ZIP/Postal code |
1090 |
| Country |
Austria |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE10001 |
Gene expression profiling in NCoR deficient mouse livers |
|
