|
| Status |
Public on Dec 31, 2017 |
| Title |
KG1a_LKB1_negative control |
| Sample type |
RNA |
| |
|
| Source name |
KG1a cells with LKB1 gene as negative control
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: KG1a cells
genotype/variation: negative control (NC)
phenotype: LKB1 gene normal expression
|
| Treatment protocol |
We use shRNA to knock down(KD) the expression of LKB1 gene at the human acute myeloid leukemia KG1a cells ,meanwhile build the negative control with normal LKB1 expression.
|
| Growth protocol |
KG1a cells were maintained in Iscove’s modified Dulbecco’s media supplemented with 10% fetal bovine serum. All of the cells were incubated at 37°C in a humidified atmosphere with 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip® PrimeView™ Human Gene Expression Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000.
|
| Data processing |
The data were analyzed with rma using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Jul 27, 2016 |
| Last update date |
Dec 31, 2017 |
| Contact name |
Huihan Wang |
| E-mail(s) |
wang.huihan@yahoo.com
|
| Organization name |
China Medical University
|
| Street address |
No. 36 Sanhao Street
|
| City |
Shenyang |
| ZIP/Postal code |
110004 |
| Country |
China |
| |
|
| Platform ID |
GPL16043 |
| Series (1) |
| GSE84904 |
Expression data for human acute myeloid leukemia KG1a cells and KG1a LKB1 gene knock down cells |
|
