|
| Status |
Public on Dec 31, 2007 |
| Title |
Gene profiling in hearts of heat stressed rats, acclimated for 1mo |
| Sample type |
RNA |
| |
|
| Source name |
heart
|
| Organism |
Rattus norvegicus |
| Characteristics |
Sabra strain, males, 3 mo old, heart
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was prepared from individual tissue samples using Tri-Reagent isolation protocol. Three pools (6 animals) were prepared for each experimental group.
|
| Label |
[32P]dATP
|
| Label protocol |
Standard Clontech protocol was used
|
| |
|
| Hybridization protocol |
The membranes were prehybridized for 1 h at 68oC in a hybridization solution (Clontech) containing 0.1 mg/ml sheared salmon testes DNA (Sigma) to block nonspecific binding. Synthesized radio labeled cDNA probe was applied to each membrane and hybridized overnight at 68oC. Cot-1 DNA (Clontech) (1 _g/ml) was added to block nonspecific binding.
|
| Scan protocol |
For analysis, the membranes were exposed for 15 and 24 h to a phosphor screen and detected using a Bio-imaging Analyzer BAS2000 (Fuji Photo Film Co. Ltd Japan.
|
| Description |
left ventricle of heart excised from rats subjected to heat stress at 41oC (2h followed by 1h recovery at 24oC, following heat acclimation (34oC, 1mo).
|
| Data processing |
We used Atlas Image 2.01 software (Clontech) to grid the phosphor image, to record the pixel density of each spot, and to perform background subtraction. The background-subtracted data were then further analyzed.
|
| |
|
| Submission date |
Jun 26, 2007 |
| Last update date |
Jul 03, 2007 |
| Contact name |
Michal Horowitz |
| E-mail(s) |
horowitz@cc.huji.ac.il
|
| Phone |
972 2 6757588
|
| Fax |
972 2 6439736
|
| Organization name |
The hebrew University
|
| Lab |
Environmental Physiology
|
| Street address |
POB 12272
|
| City |
Jerusalem |
| ZIP/Postal code |
91120 |
| Country |
Israel |
| |
|
| Platform ID |
GPL155 |
| Series (1) |
| GSE8329 |
Gene expression profiling in the heart following exposure of rats to heat acclimation, its loss and re-induction. |
|
