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Sample GSM1657356 Query DataSets for GSM1657356
Status Public on May 08, 2015
Title cbt over-expression flies, ZT3
Sample type RNA
 
Source name cbt over-expression flies, ZT3
Organism Drosophila melanogaster
Characteristics tissue: fly heads
treatment: cbt over-expression
Growth protocol Flies were entrained in LD (light: dark) condition for 3-4 days and harvested at two time points: ZT3 and ZT15.
Extracted molecule total RNA
Extraction protocol Total RNA was prepared (30 heads per sample) using Trizol reagent (Invitrogen) according to the manufacturer's protocol.
Label biotin
Label protocol RNA was then used to prepare a probe that was hybridized to Drosophila 2.0 gene expression arrays (Affymetrix) according to the manufacturer's protocol.
 
Hybridization protocol standard Affymetrix protocol
Scan protocol standard Affymetrix protocol
Data processing CEL files were imported to Affymetrix Expression Console program, and data were normalized using Robust Multichips Analysis. Differential expression was tested for probes that had expression levels above 4.0 (log2 scale) in at least one group (mutants or WT). All other probes were excluded from the analysis.
 
Submission date Apr 14, 2015
Last update date May 08, 2015
Contact name Sebastian Kadener
E-mail(s) skadener@gmail.com
Organization name Brandeis University
Street address 415 South Street
City Waltham
State/province MA
ZIP/Postal code 9190401
Country USA
 
Platform ID GPL1322
Series (1)
GSE67865 Microarray data obtained from control, cbtRNAi and cbtOE flies

Data table header descriptions
ID_REF
VALUE RMA signal

Data table
ID_REF VALUE
1634794_at 5.666797
1631805_at 2.559575
1641449_at 8.518362
1623068_at 7.408953
1631512_at 6.535621
1626427_a_at 3.407241
1629362_at 4.206566
1628406_s_at 8.505135
AFFX-BioB-3_at 4.683218
1633998_s_at 9.011708
1633684_at 9.305146
1625026_at 7.760567
1630761_at 3.992423
1628339_a_at 5.284411
1633109_at 5.711688
1633321_at 4.829862
1627287_at 8.170562
1635636_at 9.541474
1640906_at 7.496308
AFFX-Dros-18S_rRNA_M_at 7.359202

Total number of rows: 10956

Table truncated, full table size 218 Kbytes.




Supplementary file Size Download File type/resource
GSM1657356_Q03-A-3-plus.CEL.gz 1.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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