|
| Status |
Public on Mar 01, 2017 |
| Title |
metastatic tissue #1 |
| Sample type |
genomic |
| |
|
| Source name |
fresh/frozen melanoma tissue
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: fresh/frozen melanoma tissue
sample type: lymph node
|
| Growth protocol |
Human melanoma cell lines were maintained in RPMI 1640 medium supplemented with 10% foetal bovine serum, 300 mg/l L-glutamine and antibiotics. All cell lines were obtained from Coriell Cell Repositories of the Coriell Institute for Medical Research, and all chemicals were obtained from Sigma-Aldrich.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was purified using Intron G-Spin Genomic DNA Extraction kit.
|
| Label |
biotin
|
| Label protocol |
Labeling, hybridization and image scan were performed according to the recent laboratory protocol of UD-GenoMed Medical Genomic Technologies Ltd.
|
| |
|
| Hybridization protocol |
standard Affymetrix protocol
|
| Scan protocol |
standard Affymetrix protocol
|
| Data processing |
Affymetrix Genotyping Console was used for primary analysis. Copy number values were extracted and converted from CEL files into signal intensities using apt-copynumber-cyto 1.14.4 software, and CYCHP files were generated by Chromosome Analysis Suite (ChAS) 2.0 software according to the current protocol of UD-GenoMed Medical Genomic Technologies Ltd. ChAS 2.0 and Nexus 6.1 Copy Number Discovery softwares were used for further analysis.
|
| |
|
| Submission date |
Feb 03, 2015 |
| Last update date |
Mar 01, 2017 |
| Contact name |
Laura Vizkeleti |
| E-mail(s) |
laura.vizkeleti@gmail.com
|
| Organization name |
Semmelweis University
|
| Department |
2nd Deptartment of Pathology
|
| Street address |
Üllői str. 93.
|
| City |
Budapest |
| ZIP/Postal code |
1091 |
| Country |
Hungary |
| |
|
| Platform ID |
GPL18637 |
| Series (2) |
| GSE65569 |
Altered integrin expression patterns revealed by microarray in matched human melanomas (snp) |
| GSE65570 |
CNV and expression patterns in matched human melanoma tissues and cell lines with different invasive property. |
|
