|
| Status |
Public on May 01, 2015 |
| Title |
WT LPS 24 hrs |
| Sample type |
RNA |
| |
|
| Source name |
Mouse embryonic fibroblasts
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
genotype: WT
treatment: LPS 24 hrs
cell type: MEF
|
| Treatment protocol |
Mouse embryonic fibroblasts were treated at 70% confluency with Lipo-polysaccharide (LPS-1mg/ml), Lymphotoxin b receptor agonist antibody (LTbR - 0.35mg/ml) individually and in a cotreatment regime with both ligands (LPS+LTbR).
|
| Growth protocol |
Mouse embryonic fibroblasts were treated at 70% confluency.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using RNeasy Kit (QIAGEN) and on column DNase digestion was performed according to manufacturer protocol. Quality control was performed using Agilent Bioanalyzer.
|
| Label |
Cy3
|
| Label protocol |
Biotinylated cRNA were prepared with TargetAmpTM- Nano labelling Kit for illumina Expression Beadchip
|
| |
|
| Hybridization protocol |
illumina standard direct hybridization protocol
|
| Scan protocol |
By using illumina Bead Array Reader version 3.7.9.27572
|
| Data processing |
The raw data was extracted from illumina's Genome studio software and the same used to check the quality of data. The data were normalized using quantile normalisation method.
|
| |
|
| Submission date |
Oct 14, 2014 |
| Last update date |
May 01, 2015 |
| Contact name |
Balaji Banoth |
| E-mail(s) |
balajibanoth@nii.ac.in
|
| Organization name |
National Institute of Immunology
|
| Department |
Immunology
|
| Lab |
Systems Immunology Lab
|
| Street address |
Aruna Asaf Ali Marg
|
| City |
New Delhi |
| State/province |
Delhi |
| ZIP/Postal code |
110067 |
| Country |
India |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE62301 |
Developmental LTbR synergistically activates TLR4 mediated inflammatory RelA/NF-kB response |
|
