|
| Status |
Public on Aug 29, 2014 |
| Title |
A549_B+F_6h_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
A549 NSCLC cells,4nM BI-2536 and 20μM fasudil combined treat for 6 h
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: A549
treatment: 4nM BI-2536 and 20μM fasudil combined treat for 6 h
tissue: Lung
cell type: epithelial cell
genotype: KRAS mutation
disease: Carcinoma
|
| Treatment protocol |
A549 cells were treated with BI-2536 (4 nM), fasudil (20 µM), or the combination of BI-2536 and fasudil for 6 h as indicated.
|
| Growth protocol |
cells were grown at 37°C under a humidified 95:5 (%; v/v) mixture of air and CO2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned by using Affymetrix® GeneChip Command Console (AGCC) which installed in GeneChip® Scanner 3000 7G.
|
| Description |
Gene expression data from A549 cells after 4nM BI-2536 and 20μM fasudil combined treat for 6 h.
|
| Data processing |
The data were analyzed with Robust Multichip Analysis (RMA) algorithm using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Aug 28, 2014 |
| Last update date |
Aug 29, 2014 |
| Contact name |
Xiufeng Pang |
| E-mail(s) |
xfpang@bio.ecnu.edu.cn
|
| Phone |
+86-21-24206942
|
| Organization name |
East China Normal University
|
| Department |
School of Life Sciences
|
| Lab |
Institute of Biomedical Sciences
|
| Street address |
500 Dongchuan Road
|
| City |
Shanghai |
| ZIP/Postal code |
200241 |
| Country |
China |
| |
|
| Platform ID |
GPL16043 |
| Series (1) |
| GSE60887 |
gene expression profiles in KRAS mutant A549 cells after BI-2536 and fasudil combination treatment |
|
