|
Status |
Public on May 08, 2015 |
Title |
WiDr_C_1 |
Sample type |
RNA |
|
|
Source name |
WiDr human colorectal cancer cell line
|
Organism |
Homo sapiens |
Characteristics |
cell line: WiDr condition: CTRL
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted following the RNA-easy Quiagen protocol.
|
Label |
biotin
|
Label protocol |
100ng of total RNA from each sample was processed, labelled according to the Affymetrix GeneChip® Whole Transcript Sense Target Labelling Assay
|
|
|
Hybridization protocol |
Sample was hybridized to Affymetrix PrimeView array using a Affymetrix GeneChip Hybridization Oven 645
|
Scan protocol |
GeneChip was scanned using Affymetrix GeneChip Scanner 3000 7G
|
Description |
CRC cell lines Gene expression data from vehicle-treated WiDr cells
|
Data processing |
Data were analyzed using the RMA algorithm and then LIMMA was applied to calculate significant differential expression between samples. This was performed in R (v 3.0.2) with aroma.affymetrix and bioconductor packages
|
|
|
Submission date |
May 27, 2014 |
Last update date |
Oct 19, 2023 |
Contact name |
Júlia Perera-Bel |
E-mail(s) |
mardata-bu@researchmar.net
|
Organization name |
Hospital del Mar Research Institute
|
Department |
MARData-BU
|
Street address |
Doctor Aiguader, 88
|
City |
Barcelona |
ZIP/Postal code |
08003 |
Country |
Spain |
|
|
Platform ID |
GPL15207 |
Series (1) |
GSE57979 |
BRAF-induced tumorigenesis is IKKalpha dependent but NFκB independent |
|