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Status |
Public on Dec 01, 2016 |
Title |
biparental HS401 NSCs |
Sample type |
RNA |
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Source name |
biparental NSCs
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Organism |
Homo sapiens |
Characteristics |
cell line: HS401 parental state: biparental stem cell type: neural
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Treatment protocol |
Cells were differentiated into the neural lineage as described (Ahmad et al., 2012).
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Growth protocol |
hESC lines were cultured on mitomycin-C (Sigma-Aldrich) treated human foreskin fibroblasts (ATCC-LGC Standards, Wesel, Germany).
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Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted with the mirVana miRNA isolation kit (Ambion) following the manufacturer's protocol for total RNA isolation.
|
Label |
biotin
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Label protocol |
100ng of total RNA were amplified with the WT Expression Kit (Ambion), followed by ssDNA labeling with the WT Terminal Labeling Kit (Affymetrix); all steps were performed according to the manufacturers' protocols.
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Hybridization protocol |
130µl hybridization mix prepared from labeled ssDNA was hybridized for 17h at 45°C to GeneChip Human Gene 2.0 ST arrays. Microarrays were washed and stained in the Affymetrix Fluidics Station 450 using script FS450_002.
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Scan protocol |
Microarrays were scanned using a GeneChip Scanner 3000 7G (Affymetrix).
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Description |
neural biparental stem cells derived from HS401
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Data processing |
Data were preprocessed with the Expression Console v1.2.1.20 software (Affymetrix); background correction, nomalization and gene-level probeset summary were performed with the RMA sketch workflow using default parameter settings.
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Submission date |
May 23, 2014 |
Last update date |
Dec 01, 2016 |
Contact name |
Claus Juergen Scholz |
Organization name |
University Hospital Würzburg
|
Department |
Core Unit SysMed
|
Street address |
Josef-Schneider-Str. 2
|
City |
Würzburg |
ZIP/Postal code |
97080 |
Country |
Germany |
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Platform ID |
GPL16686 |
Series (1) |
GSE57932 |
Human parthenogenetic embryonic strem cell-derived neural stem cells express HLA-G. |
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