|
| Status |
Public on Dec 01, 2016 |
| Title |
parthenogenetic LLC9P ESCs |
| Sample type |
RNA |
| |
|
| Source name |
uniparental hESCs
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: LLC9P
parental state: uniparental
stem cell type: embryonic
|
| Treatment protocol |
Cells were differentiated into the neural lineage as described (Ahmad et al., 2012).
|
| Growth protocol |
hESC lines were cultured on mitomycin-C (Sigma-Aldrich) treated human foreskin fibroblasts (ATCC-LGC Standards, Wesel, Germany).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with the mirVana miRNA isolation kit (Ambion) following the manufacturer's protocol for total RNA isolation.
|
| Label |
biotin
|
| Label protocol |
100ng of total RNA were amplified with the WT Expression Kit (Ambion), followed by ssDNA labeling with the WT Terminal Labeling Kit (Affymetrix); all steps were performed according to the manufacturers' protocols.
|
| |
|
| Hybridization protocol |
130µl hybridization mix prepared from labeled ssDNA was hybridized for 17h at 45°C to GeneChip Human Gene 2.0 ST arrays. Microarrays were washed and stained in the Affymetrix Fluidics Station 450 using script FS450_002.
|
| Scan protocol |
Microarrays were scanned using a GeneChip Scanner 3000 7G (Affymetrix).
|
| Description |
embryonic uniparental stem cells derived from LLC9P
|
| Data processing |
Data were preprocessed with the Expression Console v1.2.1.20 software (Affymetrix); background correction, nomalization and gene-level probeset summary were performed with the RMA sketch workflow using default parameter settings.
|
| |
|
| Submission date |
May 23, 2014 |
| Last update date |
Dec 01, 2016 |
| Contact name |
Claus Juergen Scholz |
| Organization name |
University Hospital Würzburg
|
| Department |
Core Unit SysMed
|
| Street address |
Josef-Schneider-Str. 2
|
| City |
Würzburg |
| ZIP/Postal code |
97080 |
| Country |
Germany |
| |
|
| Platform ID |
GPL16686 |
| Series (1) |
| GSE57932 |
Human parthenogenetic embryonic strem cell-derived neural stem cells express HLA-G. |
|
