|
| Status |
Public on Mar 31, 2014 |
| Title |
WT |
| Sample type |
RNA |
| |
|
| Source name |
macrophages
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
genotype/variation: wild type
cell type: macrophages
|
| Treatment protocol |
No treatment was performed.
|
| Growth protocol |
Thioglycollate induced peritoneal macrophages were isolated from HDAC11 knockout and WT mice. The cells were cultured overnight in RPMI+10%FBS medium. Total RNA was extracted form adherent cell population.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Isolation of total RNA was performed using the Qiagen RNEasy column according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA was prepared from 100 ng of total RNA using the Ambion WT Expression Kit according to the protocol. 5.5 ug of the the resulting single-stranded 2nd cycle cDNA was labeled using the Affymetrix GeneChip WT Terminal Labeling and Hybridization Kit.
|
| |
|
| Hybridization protocol |
2.5 ug of fragmented and labeled cDNA was hybridized for 16 hr at 45C to the Affymetrix Mouse Gene 2.0 arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 using the FS450_007 wash protocol.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GCS3000 scaner.
|
| Description |
Gene expression data from freshly isolated macrophages from WT mice
|
| Data processing |
The data were analyzed with the Affymetrix Expression Console software v 1.2.1 using the RMA algorithm with default analysis settings.
|
| |
|
| Submission date |
Mar 31, 2014 |
| Last update date |
Mar 31, 2014 |
| Contact name |
Ed Seto |
| E-mail(s) |
ed.seto@moffitt.org
|
| Organization name |
H. Lee Moffitt Cancer Center
|
| Department |
Molecular Oncology
|
| Lab |
Seto Lab
|
| Street address |
12902 Magnolia Dr.
|
| City |
Tampa |
| State/province |
FL |
| ZIP/Postal code |
33612 |
| Country |
USA |
| |
|
| Platform ID |
GPL16570 |
| Series (1) |
| GSE56366 |
Loss of HDAC11 leads to reduced secretion of MCP-1 and less severe EAE |
|
