|
| Status |
Public on Jun 08, 2016 |
| Title |
HSC after culture with resting Tregs; rep 3 |
| Sample type |
RNA |
| |
|
| Source name |
HSC were cultured in the presence of resting Tregs from day 9 until day 12, then HSC were re-isolated using a MoFlow cell sorter
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: umbilical cord blood
cell type: HSC
treatment: HSC were cultured in the presence of resting Tregs from day 9 until day 12, then HSC were re-isolated using a MoFlow cell sorter
|
| Treatment protocol |
Resting or activated umbilical cord blood Tregs were added at day 9 of culture. Resting Tregs were added freshly isolated whereas activated Tregs were pre-activated for 24 h with plate bound CD3, soluble CD28 and IL-2 and washed before being added to HSC cultures.
|
| Growth protocol |
UCB HSC were cultured in the presence of resting or activated Tregs from day 9 until day 12, then HSC were re-isolated using a MoFlow cell sorter.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extraction was performed using the Rneasy mini kit (Qiagen) following manufacturer's recommendations. RNA concentration was measured using the NanoDrop 1000 Spectrophotometer (Thermo Scientific, USA).
|
| Label |
biotin
|
| Label protocol |
Standard protocol used UCL genomics facilities following Affymetrix reccomendations.
|
| |
|
| Hybridization protocol |
Standard protocol used UCL genomics facilities following Affymetrix reccomendations.
|
| Scan protocol |
Standard protocol used UCL genomics facilities following Affymetrix reccomendations.
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Feb 18, 2014 |
| Last update date |
Jun 08, 2016 |
| Contact name |
Aurore Saudemont |
| E-mail(s) |
aurore.saudemont@anthonynolan.org
|
| Organization name |
University College London
|
| Department |
Anthony Nolan Research Institute
|
| Lab |
Anthony Nolan Research Institute
|
| Street address |
Royal Free Campus, Pond Street
|
| City |
London |
| ZIP/Postal code |
NW3 2QG |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL16686 |
| Series (1) |
| GSE55091 |
Effect of resting or activated human regulatory T cells (Tregs) on human natural killer (NK) cell differentiation in vitro. |
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