|
| Status |
Public on Jan 01, 2016 |
| Title |
genes expression in esophageal squamous cell carcinoma KYSE150 cells transfected with LOXL2 △e13 |
| Sample type |
RNA |
| |
|
| Source name |
esophageal squamous cell carcinoma
|
| Organism |
Homo sapiens |
| Characteristics |
treatment: LOXL2 △e13 overexpression
cell line: KYSE150
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Data processing |
All probe signals were normalized log2 ratio, then RMA analysyis: background correction on the PM values, quantile normalization across all the chips in the experiment, Log2 transformation, median polish summarization.
|
| |
|
| Submission date |
Dec 27, 2013 |
| Last update date |
Jan 01, 2016 |
| Contact name |
Guo-Qing Lv |
| E-mail(s) |
gqlvster@gmail.com
|
| Phone |
86-754-88900413
|
| Organization name |
Department of Biochemistry and Molecular Biology
|
| Street address |
Xinling road
|
| City |
Shantou |
| State/province |
Guangdong |
| ZIP/Postal code |
515041 |
| Country |
China |
| |
|
| Platform ID |
GPL15207 |
| Series (1) |
| GSE53676 |
A novel Lysyl oxidase like 2 alternative splicing LOXL2 △e13 in esophageal squamous carcinoma cells |
|
