|
| Status |
Public on Jul 27, 2015 |
| Title |
benzene-exposed group,biological rep3 |
| Sample type |
RNA |
| |
|
| Source name |
benzene-exposed worker 3
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Peripheral blood
treatment: benzene exposed
|
| Treatment protocol |
All blood samples from each subject were centrifuged and subjected to RNA extraction.
|
| Growth protocol |
peripheral blood samples were
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using TRIzol reagent (Invitrogen, USA) according to the manufacturer’s protocol.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the protocol from the Ambion® WT Expression kit for Affymettrix® GeneChip®Whole Transcrpit (WT) Expression Arrays
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human Gene 2.0 ST Chips. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the GeneChip® Scanner 3000 7G/4 Color Early Access.
|
| Description |
LncRNA and mRNA expression data from benzene-exposed worker 3
|
| Data processing |
The data were analyzed with the affymetrix Expression Console software using Affymetrix default analysis settings and RMA as normalization method.We applied the RVM F-test to filter the differentially expressed genes. After the significance analysis and false discovery rate (FDR) analysis, we selected the differentially expressed genes according to the P value threshold. Both p-value <0.05 and FDR<0.01 were considered statistically significant.
|
| |
|
| Submission date |
Sep 10, 2013 |
| Last update date |
Jul 27, 2015 |
| Contact name |
ai aa gao |
| E-mail(s) |
gaoai0980@163.com
|
| Organization name |
School of Public Health, Capital Medical University
|
| Street address |
NO10, Xi tou tiao, Youanmen,Fengtai District
|
| City |
beijing |
| ZIP/Postal code |
100069 |
| Country |
China |
| |
|
| Platform ID |
GPL16686 |
| Series (1) |
| GSE50737 |
LncRNA and mRNA expression data involved in benzene hematotoxicity |
|
