|
| Status |
Public on Nov 01, 2013 |
| Title |
PC3 miR-95 |
| Sample type |
RNA |
| |
|
| Source name |
PC3 miR-95
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: PC3
treatment: miR-95 overexpression
|
| Growth protocol |
PC3 prostate cells stably overexpressing miR control or miR-95 were grown in 10% FBS/DMEM media in 37C humidifed incubator
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted and purified using the Qiagen RNAeasy mini kit as per manufacturer's instructions
|
| Label |
biotin
|
| Label protocol |
Labeling, hybridization, and scanning were performed at The Centre for Applied Genomics (Hospital for Sick Children)
|
| |
|
| Hybridization protocol |
standard Affymetrix protocol
|
| Scan protocol |
standard Affymetrix protocol
|
| Data processing |
Gene expression array data was normalized using the default parameters in Affymetrix Expression Console Software v 1.2
|
| |
|
| Submission date |
Aug 05, 2013 |
| Last update date |
Nov 01, 2013 |
| Contact name |
Stanley K Liu |
| Organization name |
Sunnybrook Research Institute
|
| Lab |
S207B
|
| Street address |
2075 Bayview Avenue
|
| City |
Toronto |
| State/province |
Ontario |
| ZIP/Postal code |
M4N3M5 |
| Country |
Canada |
| |
|
| Platform ID |
GPL16686 |
| Series (1) |
| GSE49559 |
Gene expression data from PC3 prostate cells overexpressing control miR or miR-95 |
|
