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Status |
Public on Aug 01, 2013 |
Title |
CHES1 rep3 |
Sample type |
RNA |
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Source name |
H1299 infected with a vector expressing CHES1, 5 days post-infection
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Organism |
Homo sapiens |
Characteristics |
cell line: H1299 vector: expressing CHES1
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Treatment protocol |
Cells were infected with an empty retrovirus or a retrovirus expressing CHES1 and selected during three days with 5 µg/ml puromycin before RNA extraction.
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Growth protocol |
Cells were cultured in Dulbecco’s modified Eagle medium (DMEM; Wisent, Montréal, QC) supplemented with 10% fetal bovine serum (FBS; Wisent) and 1% penicillin G/streptomycin sulfate (Wisent).
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Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the protocol from the Ambion® WT Expression kit for Affymettrix® GeneChip®Whole Transcrpit (WT) Expression Arrays
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Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human Gene 2.0 ST Chips. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
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Scan protocol |
GeneChips were scanned using the GeneChip® Scanner 3000 7G/4 Color Early Access.
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Description |
Gene expression data from H1299 infected with a vector expressing CHES3
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Data processing |
The data were analyzed with the affymetrix Expression Console software using Affymetrix default analysis settings and RMA as normalization method. Transcripts with a fold change higher or equal to 1.5 and a p-value˂ 0.05 according to the two-sample student's t-test were selected for final determination.
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Submission date |
Jul 31, 2013 |
Last update date |
Aug 01, 2013 |
Contact name |
Mathieu Vernier |
E-mail(s) |
mathieu.vernier@gmail.com
|
Organization name |
Université de Montréal
|
Department |
Biochemistry
|
Lab |
Dr Ferbeyre
|
Street address |
2900 Edouard Montpetit
|
City |
Montréal |
State/province |
Quebec |
ZIP/Postal code |
H3T1J4 |
Country |
Canada |
|
|
Platform ID |
GPL16686 |
Series (1) |
GSE49422 |
Expression data from H1299 human non-small cell lung carcinoma cell lines stably expressing CHES1 compared with H1299 infected with an empty vector |
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