|
| Status |
Public on Feb 03, 2014 |
| Title |
Radiation 2 |
| Sample type |
RNA |
| |
|
| Source name |
PSN1 cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: PSN1
cell type: pancreatic cancer
|
| Treatment protocol |
Cell cultures were irradiated using 320 X-ray source (Precision XRay Inc.) at a dose rate of 2.3 Gy/min.
|
| Growth protocol |
Cells were grown in RPMI with 10% FBS in an atmosphere of 5% CO2/95% air at 37°C
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Polysome bound mRNA was isolated using sucrose gradient fractionation
|
| Label |
Biotin
|
| Label protocol |
See manufacturer's protocols
|
| |
|
| Hybridization protocol |
See manufacturer's protocols
|
| Scan protocol |
See manufacturer's protocols
|
| Description |
Polysome-bound RNA
|
| Data processing |
The data were analyzed with Partek Genomics Suite. ANOVA table shows ANOVA analysis from partek for the different comparisons and lists the pvalue and fold change as well as the RMA normalized log2 transformed intensity values.
|
| |
|
| Submission date |
Jun 19, 2013 |
| Last update date |
Feb 03, 2014 |
| Contact name |
Thomas Hayman |
| E-mail(s) |
thomas.hayman@yale.edu
|
| Organization name |
Yale University
|
| Department |
Department of Therapeutic Radiology
|
| Street address |
15 York Street
|
| City |
New Haven |
| State/province |
CT |
| ZIP/Postal code |
06511 |
| Country |
USA |
| |
|
| Platform ID |
GPL15207 |
| Series (1) |
|
