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| Status |
Public on Sep 06, 2017 |
| Title |
Expression data from a human lung metastatic cell line treated with siPGC1alpha or siControl |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
To understand global expression changes in a knockdown of PGC1alpha (siPGC1alpha) vs control (siControl) in a lung metastatic cell line (4175)
Metabolic adaptations play a key role in fuelling tumour growth. However, less is known regarding the metabolic changes that promote cancer progression to metastatic disease. Herein, we reveal that PGC-1a expression and activity are differentially regulated depending on breast cancer metastatic sites. Breast cancer cells that preferentially metastasize to lung display striking upregulation of PGC-1a expression. PGC-1a promotes breast cancer cell migration and invasion in vitro and augments lung metastasis in vivo. MDA-MB-231 lung variant (4175) cells were treated with a short interfering RNA (siRNA) for human PGC-1a (PPARG1A) or control siRNA for 48hrs 24 hours post-plating cells.
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| Overall design |
MDA-MB-231 lung variant (4175) cells were treated with a short interfering RNA (siRNA) for human PGC-1a (PPARG1A) or control siRNA for 48hrs 24 hours post-plating cells.
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| Contributor(s) |
Andrzjewski S, Johnson RM, Tabariès S, Annis MG |
| Citation(s) |
28988825 |
| Submission date |
Jun 01, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Julie St-Pierre |
| E-mail(s) |
julie.st-pierre@mcgill.ca
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| Organization name |
McGill University
|
| Department |
Department of Biochemistry
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| Street address |
3655 Promenade Sir William Osler
|
| City |
Montreal |
| State/province |
Quebec |
| ZIP/Postal code |
H3G 1Y6 |
| Country |
Canada |
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| Platforms (1) |
| GPL23159 |
[Clariom_S_Human] Affymetrix Clariom S Assay, Human (Includes Pico Assay) |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE99557 |
Expression data from a lung metastatic cell line or metastatic explants in mouse and human |
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| Relations |
| BioProject |
PRJNA388962 |
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