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| Status |
Public on Jan 30, 2004 |
| Title |
Gene Expression-Based High Throughput Screening: HL-60 Cells Treated with ATRA and PMA |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
We developed a general approach to small molecule library screening called GE-HTS (Gene Expression-Based High Throughput Screening) in which a gene expression signature is used as a surrogate for cellular states and applied it to the identification of compounds inducing the differentiation of acute myeloid leukemia cells. In screening 1,739 compounds, we identified 8 that reliably induced the differentiation signature, and furthermore yielded functional evidence of bona fide differentiation.
This data set contains HL-60 cells treated in a time course, in replicate, with 1 uM all trans retinoic acid (ATRA) and 10 nM phorbol 12-myristate 13-acetate (PMA). Also included are untreated HL-60 controls. This data set was used to select marker genes that distinguish the undifferentiated from the PMA or ATRA differentiated states.
Keywords = AML
Keywords = leukemia
Keywords = HL-60
Keywords = chemical genomics
Keywords: repeat sample
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| Contributor(s) |
Stegmaier K, Ross KN, Colavito SA, O'Malley S, Stockwell BR, Golub TR |
| Citation(s) |
14770183 |
| Submission date |
Jan 21, 2004 |
| Last update date |
Jul 08, 2016 |
| Contact name |
Ken Ross |
| E-mail(s) |
kross@broadinstitute.org
|
| Phone |
617-714-7473
|
| Fax |
617-714-8955
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| URL |
http://www.broad.mit.edu/cancer/
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| Organization name |
Broad Institute
|
| Department |
Cancer Genomics
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| Lab |
Golub Lab
|
| Street address |
7 Cambridge Center
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| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02142 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL80 |
[Hu6800] Affymetrix Human Full Length HuGeneFL Array |
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| Samples (21)
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| This SubSeries is part of SuperSeries: |
| GSE995 |
Differentiation of acute myeloid leukemia cells |
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| Relations |
| BioProject |
PRJNA89877 |
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