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| Status |
Public on Mar 21, 2017 |
| Title |
Drosophila CNS OXPHOS inhibition microarray |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by array
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| Summary |
Mitochondrial dysfunction causes biophysical, metabolic and signalling changes that alter homeostasis and reprogram cells. We used a Drosophila model in which individual subunits of 4 OXPHOS complexes are knocked-down in neurons in the larval CNS.
We used microarray analysis to investigate gene expression changes caused by knock down of OXPHOS subunits of complexes I, III, IV and V in neurons.
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| Overall design |
RNA was purified from the late third instar larval CNS from control larvae (nSyb-Gal4/w), or larvae expressing RNAi transgenes targeting ND-75 (complex I, RNAi line #33910), UQCR-14 (complex III, RNAi line #109542), COX5B (complex IV, RNAi line #105769) and ATPsynCf6 (complex V, RNAi line #107826) in neurons using nSyb-Gal4. Three replicates are included for the each condition.
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| Contributor(s) |
Bateman JM, Duncan OF |
| Citation missing |
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| Submission date |
Mar 20, 2017 |
| Last update date |
May 04, 2018 |
| Contact name |
Joseph M. Bateman |
| E-mail(s) |
joseph_matthew.bateman@kcl.ac.uk
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| Phone |
2078488144
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| Organization name |
King's College London
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| Street address |
Guy's campus
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| City |
London |
| State/province |
(Click to Select US State) |
| ZIP/Postal code |
SE1 1UL |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL1322 |
[Drosophila_2] Affymetrix Drosophila Genome 2.0 Array |
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| Samples (15)
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| Relations |
| BioProject |
PRJNA379770 |
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