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| Status |
Public on Jun 01, 2017 |
| Title |
KLF family members KLF4 and KLF7 regulate corneal epithelium [siKLF4-siKLF7_differentiation] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
While the regulatory landscape during stem cell differentiation has been well characterized, the shared and unique regulatory mechanisms in different ectodermally-derived epithelial cells have not been well described. Through defining the complement of super enhancers and typical enhancers in corneal epithelium for the first time, we show that regulatory regions are often shared between cell types of the same lineage, and that corneal super enhancers are already marked as potential regulatory domains in embryonic stem cells. Through the enrichment of KLF motifs in enhancers, we identified and defined a novel role for Kruppel family member KLF7 in promoting the corneal progenitor cell state, in many cases working antagonistically to corneal differentiation promoting KLF4. Our work highlights the importance of balance between proliferation and differentiation, both for proper tissue development and for homeostasis.
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| Overall design |
pools of siRNAs were used to knockdown KLF4 or KLF7 in duplicate, with 2 scramble siRNA control cell plates. RNA was extracted and submitted for microarray.
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| Contributor(s) |
Herndon Klein R, Andersen B |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Feb 03, 2017 |
| Last update date |
Mar 15, 2019 |
| Contact name |
Bogi Andersen |
| Organization name |
University of California, Irvine
|
| Department |
Medicine
|
| Lab |
Andersen
|
| Street address |
839 Health Sciences Dr.
|
| City |
Irvine |
| State/province |
CA |
| ZIP/Postal code |
92697 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL16686 |
[HuGene-2_0-st] Affymetrix Human Gene 2.0 ST Array [transcript (gene) version] |
|
| Samples (6)
|
| GSM2476185 |
scramble control replicate 1 HCE [siKLF4-siKLF7_differentiation] |
| GSM2476186 |
scramble control replicate 2 HCE [siKLF4-siKLF7_differentiation] |
| GSM2476187 |
siKLF4 replicate 1 HCE [siKLF4-siKLF7_differentiation] |
| GSM2476188 |
siKLF4 replicate 2 HCE [siKLF4-siKLF7_differentiation] |
| GSM2476189 |
siKLF7 replicate 1 HCE [siKLF4-siKLF7_differentiation] |
| GSM2476190 |
siKLF7 replicate 2 HCE [siKLF4-siKLF7_differentiation] |
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| This SubSeries is part of SuperSeries: |
| GSE94472 |
KLF family members KLF4 and KLF7 regulate corneal epithelium |
|
| Relations |
| BioProject |
PRJNA369795 |
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