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| Status |
Public on Oct 25, 2008 |
| Title |
Expression data from human embryonic stem cell differentiation |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
MicroRNAs (miRNAs) are noncoding RNAs of approximately 22 nucleotides in length that usually suppress the translation of target messenger RNAs (mRNAs) through partial complementarity to the 3¡¦ untranslated region (3¡¦ UTR) of protein-coding mRNAs in animals. However, there is increasing evidence that miRNAs can also reduce the steady-state levels of their target mRNAs in animals. In this investigation, both miRNA and mRNA profiles from the undifferentiated human embryonic stem cell line hES-T3 (T3ES), hES-T3 derived embryoid bodies (T3EB) and hES-T3 differentiated fibroblast-like cells (T3DF) were quantitatively determined. Three common target genes of three highly expressed hES cell-specific miRNAs were identified by inverse expression levels of miRNAs to their target mRNAs.
Keywords: cell type comparison
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| Overall design |
Three types of human embryonic stem cells and their derived cells (the undifferentiated human embryonic stem cell line hES-T3 (T3ES), hES-T3 derived embryoid bodies (T3EB) and hES-T3 differentiated fibroblast-like cells (T3DF)) were selected for RNA extraction. The genome-wide mRNA expression of T3ES, T3EB and T3DF cells was determined using Affymetrix human genome U133 plus 2.0 GeneChip. The abundantly expressed genes (10-fold above overall mean) in T3ES, T3EB and T3DF cells are selected to compare with the predicted gene targets of differentially expressed miRNAs in the same set of cells.
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| Contributor(s) |
Li SS, Yu S, Kao L, Tsai Z, Singh S, Chen B, Yang P |
| Citation(s) |
19229866 |
| Submission date |
Oct 26, 2007 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Sung-Liang Yu |
| E-mail(s) |
slyu@ntu.edu.tw
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| Phone |
886-2-23958341
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| Organization name |
National Taiwan University
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| Department |
Clinical Laboratory Sciences and Medical Biotechnology
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| Lab |
Microarray Core Facility
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| Street address |
Jen Ai Road Section1
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| City |
Taipei |
| ZIP/Postal code |
100 |
| Country |
Taiwan |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (8)
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| GSM239824 |
Human embryonic stem cell line T3ES, biological rep1 |
| GSM239825 |
Human embryonic stem cell line T3ES, biological rep2 |
| GSM239826 |
Human embryonic stem cell line T3ES, biological rep3 |
| GSM239827 |
hES-T3 derived embryoid bodies, biological rep1 |
| GSM239828 |
hES-T3 derived embryoid bodies, biological rep2 |
| GSM239829 |
Human fibroblast-like cells, biological rep1 |
| GSM239830 |
Human fibroblast-like cells, biological rep2 |
| GSM239831 |
Human fibroblast-like cells, biological rep3 |
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| Relations |
| BioProject |
PRJNA103197 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE9440_RAW.tar |
37.6 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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