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| Status |
Public on Oct 08, 2004 |
| Title |
Pseudomonas aeruginosa infection of Calu-3 human lung epithelial cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
This series contains 5 groups of U133A arrays with targets from Calu-3 cell line post-infection with P aeruginosa strains. Calu-3 human lung epithelial cells were seeded onto 6-well plates and grown to about 80% confluency. Epithelial monolayers were infected with 108 CFUs per ml of each bacterial strain for 60 min and then washed extensively with phosphate-buffered saline. The cultures were subsequently incubated in fresh RPMI 1640 medium supplemented with 10% fetal bovine serum and 100 µg/ml gentamicin. After 6 h, total RNA from the cells was prepared with RNAzol and further purified with RNEasy for microarray hybridization. All arrays processed and globally scaled to 500 using MAS 5.
CONTROL (4 arrays) - no infection
FRD1 (4 arrays)
FRD1234 (3 arrays)
FRD440 (4 arrays)
FRD875 (4 arrays)
Keywords = Cystic Fibrosis
Keywords = Pseudomonas aeruginosa
Keywords = CF
Keywords = flagellin
Keywords = alginate
Keywords = lung
Keywords: repeat sample
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| Contributor(s) |
Cobb LM, Mychaleckyj JC, Wozniak DJ, López-Boado YS |
| Citation(s) |
15494517 |
| Submission date |
Dec 19, 2003 |
| Last update date |
Aug 10, 2018 |
| Contact name |
Josyf Mychaleckyj |
| E-mail(s) |
jmychale@wfubmc.edu
|
| Phone |
336-713-7540
|
| Organization name |
Wake Forest Univ School of Medicine
|
| Department |
Center for Human Genomics
|
| Lab |
MYCHALECKYJ
|
| Street address |
|
| City |
Winston-Salem |
| State/province |
NC |
| ZIP/Postal code |
27157 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL96 |
[HG-U133A] Affymetrix Human Genome U133A Array |
|
| Samples (19)
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| Relations |
| BioProject |
PRJNA87945 |
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