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| Status |
Public on May 31, 2017 |
| Title |
Prosurvival long noncoding RNA PINCR regulates a subset of p53 targets in colorectal cancer cells by binding to Matrin 3 [Affymetrix Human Gene 2.0 ST] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Thousands of long noncoding RNAs (lncRNAs) have been discovered, yet the function of the vast majority remains unclear. Here, we show that a p53-regulated lncRNA which we named PINCR (p53-induced noncoding RNA), is induced ~100-fold after DNA damage and exerts a critical prosurvival function in colorectal cancer cells (CRC) in vitro and tumor growth in vivo. Targeted deletion of PINCR in CRC cells significantly impaired G1 arrest and induced hypersensitivity to chemotherapeutic drugs. PINCR regulates the induction of a subset of p53 targets involved in G1 arrest and apoptosis, including BTG2, RRM2B and GPX1. Using a novel RNA pulldown approach that utilized endogenous S1-tagged PINCR, we show that PINCR associates with the promoters of these genes by binding to RNA-binding protein Matrin 3 that, in turn, associates with p53. Our findings uncover a critical prosurvival function of a p53/PINCR/Matrin 3 axis in response to DNA damage in CRC cells.
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| Overall design |
HCT116 p53 WT, SW48 p53 WT and RKO p53 WT cells were kept untreated or treated with Nutlin-3a and RNA samples were collected in duplicates.
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| Contributor(s) |
Chaudhary R, Subramanian M, Li XL, Zhu YJ, Lal A |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Nov 18, 2016 |
| Last update date |
Mar 15, 2019 |
| Contact name |
Yuelin Jack Zhu |
| E-mail(s) |
zhujack@mail.nih.gov
|
| Phone |
(240)760-7439
|
| Organization name |
NCI
|
| Department |
Genetics Branch, Center for Cancer Research
|
| Lab |
Dr. Paul Meltzer's Lab
|
| Street address |
9000 Rockville Pike
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL16686 |
[HuGene-2_0-st] Affymetrix Human Gene 2.0 ST Array [transcript (gene) version] |
|
| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE90086 |
Prosurvival long noncoding RNA PINCR regulates a subset of p53 targets in colorectal cancer cells by binding to Matrin 3 |
|
| Relations |
| BioProject |
PRJNA354547 |
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