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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Aug 09, 2007 |
| Title |
Transcriptional Profiling of the Megabladder Mouse - A Unique Model of Bladder Dysmorphogenesis |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Recent studies in our lab have identified a mutant mouse model of obstructive nephropathy designated mgb for megabladder. Homozygotic mgb mice (mgb-/-) develop lower urinary tract obstruction in utero due to a lack of bladder smooth muscle differentiation. This defect is the result of a random transgene insertion into chromosome 16 followed by a translocation of this fragment into chromosome 11. In an effort to identify potential gene targets affected in mgb mice, we performed transcriptional profiling on embryonic day 15 (E15) mgb-/- bladders using both a Chromosome 11/16 Custom GeneChip Array and the Affymetrix Mouse Genome 430 2.0 GeneChip. This analysis identified no definitive mis-expressed gene targets on chromosome 11. In contrast, mgb-/- mice significantly over-expressed a cluster of gene products located on the translocated fragment of chromosome 16 including urotensin II-related peptide (Urp), which was shown to be preferentially over-expressed in developing mgb-/- bladders. Immunohistochemical studies indicated that the spatial distribution of Urp was altered in mgb-/- bladders, while biochemical studies suggested a potential role for Urp in modifying smooth muscle cell phenotype in vitro. Pathway analysis of mgb microarray data showed dysregulation of at least 60 gene products associated with the differentiation of smooth muscle. In conclusion, the results of this study indicate that the molecular pathways controlling normal smooth muscle development are severely altered in mgb-/- bladders, and provide the first evidence that Urp may play a critical role in bladder smooth muscle development.
Keywords: mgb mutant bladders
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| Overall design |
Gene expression profiling was performed on two separate study groups. First, total cellular RNA was isolated from wildtype (n=2; 1 female/1 male), mgb+/- (n=2; 1 female/1 male), and mgb-/- (n=2; 1 female/1 male) E15 whole embryos. Second, total cellular RNA was isolated from the bladders of E15 wildtype (n=7; 3 female/4 male), mgb+/- mice (n=7; 3 female/4 male), and mgb-/- mice (n=11; 7 females/4 males).
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| Contributor(s) |
Singh S, Robinson M, Ismail I, Saha M, Auer H, Kornacher K, Robinson ML, Bates CM, McHugh KM |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Aug 07, 2007 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Kirk M McHugh |
| E-mail(s) |
mchughk@ccri.net
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| Phone |
614-355-2817
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| Organization name |
Columbus Children's Hospital
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| Department |
Cell & Developmental Biology
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| Street address |
700 Children's Drive
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| City |
Columbus |
| State/province |
OH |
| ZIP/Postal code |
43205 |
| Country |
USA |
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| Platforms (2) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
| GPL5722 |
Affymetrix Mus musculus 4K chromosome 11 and 16 CustomExpress Array |
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| Samples (36)
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| GSM215759 |
mgb -/- female, whole E15 embryo (HMF_529) |
| GSM215760 |
mgb -/- male, whole E15 embryo (HMM_533) |
| GSM215761 |
mgb +/- female, whole E15 embryo (HTF_523) |
| GSM215762 |
mgb +/- male, whole E15 embryo (HTM_532) |
| GSM215763 |
Wild type female, whole E15 embryo (WTF_528) |
| GSM215764 |
Wild type male, whole E15 embryo (WTM_525) |
| GSM215770 |
mgb -/- female, whole E15 embryo (F379) |
| GSM215771 |
mgb +/- female, whole E15 embryo (F382) |
| GSM215772 |
Wild type female, whole E15 embryo (F384) |
| GSM215773 |
mgb -/- male, whole E15 embryo (HM407) |
| GSM215774 |
mgb +/- male, whole E15 embryo (HT409) |
| GSM215775 |
Wild type male, whole E15 embryo (WT410) |
| GSM215776 |
mgb -/- female, E15 bladder (HMF_436) |
| GSM215777 |
mgb -/- female, E15 bladder (HMF_438) |
| GSM215778 |
mgb -/- female, E15 bladder (HMF_440) |
| GSM215779 |
mgb -/- female, E15 bladder (HMF_456) |
| GSM215780 |
mgb -/- female, E15 bladder (HMF_467) |
| GSM215781 |
mgb -/- female, E15 bladder (HMF_529) |
| GSM215782 |
mgb -/- female, E15 bladder (HMF_535) |
| GSM215783 |
mgb -/- male, E15 bladder (HMM_434) |
| GSM215784 |
mgb -/- male, E15 bladder (HMM_521) |
| GSM215785 |
mgb -/- male, E15 bladder (HMM_524) |
| GSM215786 |
mgb -/- male, E15 bladder (HMM_533) |
| GSM215787 |
mgb +/- female, E15 bladder (HTF_458) |
| GSM215788 |
mgb +/- female, E15 bladder (HTF_464) |
| GSM215789 |
mgb +/- female, E15 bladder (HTF_523) |
| GSM215790 |
mgb +/- male, E15 bladder (HTM_439) |
| GSM215791 |
mgb +/- male, E15 bladder (HTM_441) |
| GSM215792 |
mgb +/- male, E15 bladder (HTM_459) |
| GSM215793 |
mgb +/- male, E15 bladder (HTM_532) |
| GSM215794 |
Wild type female, E15 bladder (WTF_457) |
| GSM215795 |
Wild type female, E15 bladder (WTF_462) |
| GSM215796 |
Wild type female, E15 bladder (WTF_528) |
| GSM215797 |
Wild type male, E15 bladder (WTM_437) |
| GSM215798 |
Wild type male, E15 bladder (WTM_465) |
| GSM215799 |
Wild type male, E15 bladder (WTM_525) |
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| Relations |
| BioProject |
PRJNA101957 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE8715_430.2_CHIP_ALL_GENOTYPES.xls |
16.1 Mb |
(ftp)(http) |
XLS |
| GSE8715_CUSTOM 11_16_BLADDER_ARRAY_ALL_GENOTYPES_FINAL_012907.xls |
2.8 Mb |
(ftp)(http) |
XLS |
| GSE8715_CUSTOM 11_16_WHOLE_EMBRYO_ARRAY_-_FEMALE_copy.xls |
1.5 Mb |
(ftp)(http) |
XLS |
| GSE8715_CUSTOM 11_16_WHOLE_EMBRYO_ARRAY_-_MALE_copy.xls |
1.5 Mb |
(ftp)(http) |
XLS |
| GSE8715_RAW.tar |
34.6 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
| Processed data are available on Series record |
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