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| Status |
Public on Jul 17, 2007 |
| Title |
Genes in nonpermissive temperature-induced cell growth arrest and differentiation of tracheal epithelial RTEC11 cells |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
|
| Summary |
We performed global scale microarray analysis to identify detailed mechanisms by which nonpermissive temperature induces cell growth arrest and differentiation in tracheal epithelial RTEC11 cells harboring temperature-sensitive simian virus 40 large T-antigen by using an Affymetrix GeneChip system. Tracheal epithelial RTEC11 cells used in this study were derived from transgenic rats harboring a temperature-sensitive simian virus 40 large T-antigen. Although the cells grew continuously at the permissive temperature, the nonpermissive temperature led to cell growth arrest and differentiation.
Keywords: rat tracheal epithelial RTEC11; growth arrest; differentiation; gene expression
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| Overall design |
A rat conditionally immortalized tracheal epithelial cell line, RTEC11, derived from transgenic rats harboring a temperature-sensitive simian virus 40 large T-antigen was used. RTEC11 cells were cultured at permissive (33°C) or nonpermissive (39°C) temperature for 3 days.
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| Web link |
http://www.lsrc.u-toyama.ac.jp/mgrc/html/laboratory.html
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| |
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| Contributor(s) |
Tabuchi Y, Doi T, Takasaki I |
| Citation(s) |
18725308 |
| Submission date |
Jul 11, 2007 |
| Last update date |
Mar 03, 2017 |
| Contact name |
Yoshiaki Tabuchi |
| E-mail(s) |
ytabu@cts.u-toyama.ac.jp
|
| Organization name |
University of Toyama
|
| Department |
Life Science Research Center
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| Lab |
Division of Molecular Genetics Research
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| Street address |
2630 Sugitani
|
| City |
Toyama |
| State/province |
Toyama |
| ZIP/Postal code |
930-0194 |
| Country |
Japan |
| |
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| Platforms (1) |
| GPL341 |
[RAE230A] Affymetrix Rat Expression 230A Array |
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| Samples (4)
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| GSM209885 |
RTEC11 cells cultured at permissive temperature for 3 days-1 |
| GSM209886 |
RTEC11 cells cultured at permissive temperature for 3 days-2 |
| GSM209887 |
RTEC11 cells cultured at nonpermissive temperature for 3 days-1 |
| GSM209888 |
RTEC11 cells cultured at nonpermissive temperature for 3 days-2 |
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| Relations |
| BioProject |
PRJNA101517 |
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