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| Status |
Public on Aug 07, 2007 |
| Title |
resting and IL2 activated human natural killer cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
The aim of the study was to investigate the activation of human NK cells by IL2 through analyzing the global gene expression at different time points (0, 2, 8 and 24 hours) after culture with the cytokine IL2 at 100 IU/ml. NK cells with the CD56+/CD16+ and CD3- phenotype were negatively selected by immunomagnetic beads and re-examined by flow-cytometry to ensure greater than 90% purity .
Keywords: resting and IL2 activated NK cells(time series)
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| Overall design |
RNA was extracted and pooled from 4 different donors, amplified and labled according to the manufacturer’s instruction (GeneChipU133plus2® , Affymetrix Inc, CA).
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| Contributor(s) |
Dybkaer K, Iqbal J, Zhou G, Geng H, Xiao L, Schmitz A, d’Amore F, Chan WC |
| Citation(s) |
17623099 |
| Submission date |
Jun 07, 2007 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Javeed Iqbal |
| E-mail(s) |
jiqbal@unmc.edu
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| Phone |
402-559-7753
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| Organization name |
University of Nebraska Medical Center
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| Department |
Pathology and Microbiology
|
| Lab |
Center for Research in Leukemia and Lymphoma
|
| Street address |
40th street
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| City |
omaha |
| State/province |
NE |
| ZIP/Postal code |
68195 |
| Country |
USA |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA100891 |
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