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| Status |
Public on Jun 30, 2007 |
| Title |
Effect of growth in TSA or SCFA on gene expression in E. histolytica |
| Organism |
Entamoeba histolytica |
| Experiment type |
Expression profiling by array
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| Summary |
In order to dtermine the role of histone actylation in gene expresssion, E. histolytica 200:NIH trophozoites were treated with the HDAC inhibitor Trichostatin A, or grown in short chain fatty acids, which have been shown to alter histone actylation patterns in Entamoeba species.
Keywords: Expression
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| Overall design |
E. histolytica 200:NIH trophozoites grown in standard media (TYI or TYI-low glucose) were compared to trophozoites grown in TYI-LG + Trichostatin A (150 or 300 nM) for 16 or 72 hours, or in TYI-LG + short chain fatty acids (70 mM sodium acetate, 20 mM sodium propionate and 10 mM sodium butyrate). Differences in gene expression were assayed by hybridization to a full genome Affymetrix array.
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| Contributor(s) |
Ehrenkaufer GM, Eichinger DJ, Singh U |
| Citation(s) |
17612405 |
| Submission date |
Jun 07, 2007 |
| Last update date |
Mar 17, 2012 |
| Contact name |
Gretchen Marie Ehrenkaufer |
| E-mail(s) |
gehrenk@stanford.edu
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| Organization name |
Stanford Uninversity
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| Department |
Microbiology and Immunology
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| Lab |
Singh
|
| Street address |
Grant S-143, 300 Pasteur Dr.
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| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
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| Platforms (1) |
| GPL4622 |
Entamoeba histolytica E_his-1a520285F array (coding regions) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA100871 |
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