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| Status |
Public on Jun 03, 2008 |
| Title |
CD34 Overexpression |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
In order to investigate the role of CD34 antigen in haematopoietic commitment, we overexpressed the human CD34 cDNA in human CD34+ cells by retroviral gene transfer.
Keywords: treatment comparison
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| Overall design |
In a set of 10 independent experiments, gene transfer efficiency, assessed by flow cytometry analysis of ΔLNGFR positivity, ranged 15 to 30%. Transduced cells were than purified for NGFR expression at day 3 of liquid culture with the retroviral vector. Phenotypic analysis, performed on transduced/NGFR purified cells evidenced that the CD34 transgene was highly expressed at the protein level (80% of LCD34IΔN vs 30% of controls at 5 days post-infection) up to 14 days of liquid culture.
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| Contributor(s) |
Zini R, Salati S, Bianchi E, Testa A, Mavilio F, Manfredini R, Ferrari S |
| Citation(s) |
18192237 |
| Submission date |
Jun 04, 2007 |
| Last update date |
Aug 10, 2018 |
| Contact name |
Rossella Manfredini |
| E-mail(s) |
manfredini.rossella@unimore.it
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| Phone |
+390592058065
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| Organization name |
Centre for Regenerative Medicine
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| Department |
Life Sciences
|
| Street address |
Via Gottardi 100
|
| City |
Modena |
| ZIP/Postal code |
41100 |
| Country |
Italy |
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| Platforms (1) |
| GPL96 |
[HG-U133A] Affymetrix Human Genome U133A Array |
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| Samples (3) |
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| This SubSeries is part of SuperSeries: |
| GSE8040 |
CD34 antigen role in haematopoietic commitment |
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| Relations |
| BioProject |
PRJNA105389 |
