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| Status |
Public on Mar 30, 2016 |
| Title |
Expression data from human placental derived mesenchymal stem cells (PDMSCs) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
When PDMSCs were induced to heptocytes in vitro, cells mophology, stem cell markers, mitochondrial metabolism will change according to the differentiated status.But dedifferentiation reverses differentiated cells to a more primitive phenotype and PDMSCs will retain the multilineage potency. Furthermore, it will leads to the alteration of gene expression pattern.
We used microarrays to detail the global programme of gene expression underlying dedifferentiation and hepatogenic differentiation prcocesses, we intend to identify distinct classes of differentiated genes during these processes.
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| Overall design |
Human PDMSCs at passage 5 were induced to hepatocytes for 11 days, then the inductive medium was replaced by general culture medium for 1 day. Then human PDMSCs, hepatogenic PDMSCs at 11 days, dedifferentiated PDMSCs were selected for RNA extraction and hybridization on Affymetrix microarrays. To that end, we hand-selected cells at three time-points: before hepatogenic induction (P), hepatogenic PDMSCs at 11 days (H) and dedifferentiated PDMSCs for 1 day (DH) .
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| Contributor(s) |
Hu C, Yu Y |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Mar 29, 2016 |
| Last update date |
Aug 23, 2018 |
| Contact name |
Chenxia Hu |
| E-mail(s) |
11318093@zju.edu.cn
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| Organization name |
Zhejiang University
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| Department |
First Affiliated Hospital; Zhejiang University
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| Street address |
School of Medicine; First Affiliated Hospital; Zhejiang University
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| City |
Hangzhou |
| State/province |
Zhejiang |
| ZIP/Postal code |
571 |
| Country |
China |
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| Platforms (1) |
| GPL15207 |
[PrimeView] Affymetrix Human Gene Expression Array |
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| Samples (9)
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| GSM2101193 |
Hepatogenic PDMSCs for 11 days, biological rep1 |
| GSM2101194 |
Hepatogenic PDMSCs for 11 days, biological rep2 |
| GSM2101195 |
Hepatogenic PDMSCs for 11 days, biological rep3 |
| GSM2101196 |
Dedifferentiated PDMSCs for 1 day, biological rep1 |
| GSM2101197 |
Dedifferentiated PDMSCs for 1 day, biological rep2 |
| GSM2101198 |
Dedifferentiated PDMSCs for 1 day, biological rep3 |
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| Relations |
| BioProject |
PRJNA316719 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE79693_RAW.tar |
16.9 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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