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| Status |
Public on May 31, 2016 |
| Title |
(CTA) expression after exposure to 5-aza-2'-deoxycytidine in HCT116 cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
The expression of CTAs is normally restricted to gametogenic tissue but is often reactivated in the tumorigenic setting. This tumorigenic reactivation is thought to be due to global demethylation events during tumorigenesis. To test this, we employed an affymetrix microarray in the hypermethylated colorectal carcinoma tumor-derived cell line, HCT116, following 5-aza-2'-deoxycytidine treatment.
We used microarray analysis to uncover genes whose expression is modulated by DNA methylation in the colorectal carcinoma tumor derived cell line, HCT116.
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| Overall design |
HCT116 cells were exposed to 5-aza-2'-deoxycytidine for 72 hours. Total RNA was isolated and hybridized on to Affymetrix microarrays.
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| Contributor(s) |
Whitehurst AW |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Mar 09, 2016 |
| Last update date |
Nov 08, 2016 |
| Contact name |
Min Soo Kim |
| E-mail(s) |
mins.kim@utsouthwestern.edu
|
| Organization name |
University of Texas Southwestern
|
| Department |
Department of Clinical Sciences
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| Lab |
Quantitative Biomedical Research Center
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| Street address |
5323 Harry Hines Blvd.
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| City |
Dallas |
| State/province |
Texas |
| ZIP/Postal code |
75390 |
| Country |
USA |
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| Platforms (1) |
| GPL11532 |
[HuGene-1_1-st] Affymetrix Human Gene 1.1 ST Array [transcript (gene) version] |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA314780 |
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