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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Jul 27, 2009 |
| Title |
Fat Treg cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Comparisons of global gene-expression profiles revealed a greater distinction between CD4+ Treg cells and CD4+ conventional (Tconv) T cells residing in abdominal (epidydimal) fat versus in more standard locations such as the spleen, thymus and LN.
Keywords: Cell population comparison
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| Overall design |
All gene expression profiles were obtained from highly purified T cell populations sorted by flow cytometry. To reduce variability, cells from multiple mice were pooled for sorting, and three replicates were generated for all groups. RNA from 3-5 x 104 cells was amplified, labeled, and hybridized to Affymetrix M430v2 microarrays. Raw data were preprocessed with the RMA algorithm in GenePattern, and averaged expression values were used for analysis.
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| Contributor(s) |
Feuerer M, Benoist C, Mathis D |
| Citation(s) |
19633656 |
| Submission date |
May 18, 2007 |
| Last update date |
Feb 11, 2019 |
| Contact name |
CBDM Lab |
| E-mail(s) |
cbdm@hms.harvard.edu
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| Phone |
617-432-7747
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| Organization name |
Harvard Medical School
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| Department |
Microbiology and Immunobiology
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| Lab |
CBDM
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| Street address |
77 Avenue Louis Pasteur
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| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (18)
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| GSM176899 |
TconvLN, rep1 |
| GSM180519 |
TregThy, rep2 |
| GSM180531 |
TregThy, rep3 |
| GSM180533 |
TconvThy, rep2 |
| GSM180536 |
TconvThy, rep3 |
| GSM180538 |
TregLN, rep2 |
| GSM180540 |
TregLN, rep3 |
| GSM180542 |
TconvLN, rep2 |
| GSM180544 |
TconvLN, rep3 |
| GSM190555 |
Fat CD4 Treg cells, repl. 1 |
| GSM190556 |
Fat CD4 Treg cells, repl. 2 |
| GSM190562 |
Fat CD4 Treg cells, repl. 3 |
| GSM190563 |
Fat CD4 Tconv cells, repl. 1 |
| GSM190564 |
Fat CD4 Tconv cells, repl. 2 |
| GSM190565 |
Fat CD4 Tconv cells, repl. 3 |
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| Relations |
| BioProject |
PRJNA99989 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE7852_RAW.tar |
66.1 Mb |
(http)(custom) |
TAR (of CEL) |
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