|
| Status |
Public on Sep 11, 2015 |
| Title |
Effects of dexamethasone on gene expression in rat pancreatic AR42J cell line |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
|
| Summary |
The rat pancreatic cell line AR42J is relatively undifferentiated under normal culture conditions. When the glucocorticoid dexamethasone is added to the medium the cells display a dramatic decrease in proliferative rate and are induced to a more exocrine phenotype that includes increased expression of exocrine pancreas products (digestive enzymes) and more developed regulated secretion. We used microarray to determine changes in gene expression comparing control (without dexamethasone) vs induced (plus dexamethasone).
|
| |
|
| Overall design |
Three independent pairs of control and dexamethasone-induced cultures were prepared followed by total RNA extraction and microarray analysis using the Affymetrix Rat 230A chip.
|
| |
|
| Contributor(s) |
De Lisle RC, Ziemer D |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Sep 10, 2015 |
| Last update date |
Mar 03, 2017 |
| Contact name |
Robert C De Lisle |
| E-mail(s) |
rdelisle@kumc.edu
|
| Phone |
913-588-2742
|
| Organization name |
Univ Kansas Med Ctr
|
| Department |
Anatomy & Cell Biology
|
| Street address |
3901 Rainbow Blvd
|
| City |
Kansas City |
| State/province |
KS |
| ZIP/Postal code |
66160 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL341 |
[RAE230A] Affymetrix Rat Expression 230A Array |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA295306 |
Less...
More...