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| Status |
Public on Sep 30, 2015 |
| Title |
Comparative “-omics” in Mycoplasma pneumoniae Clinical Isolates Reveals Key Virulence Factors |
| Organism |
Mycoplasmoides pneumoniae |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The human respiratory tract pathogen M. pneumoniae is one of the best characterized minimal bacterium. Until now, two main groups of clinical isolates of this bacterium have been described (types 1 and 2), differing in the sequence of the P1 adhesin gene. Here, we have sequenced the genomes of 23 clinical isolates of M. pneumoniae. Studying SNPs, non-synonymous mutations, indels and genome rearrangements of these 23 strains and 4 previously sequenced ones, has revealed new subclasses in the two main groups, some of them being associated with the country of isolation. Integrative analysis of in vitro gene essentiality and mutation rates enabled the identification of several putative virulence factors and antigenic proteins; revealing recombination machinery, glycerol metabolism and peroxide production as possible factors in the genetics and physiology of these pathogenic strains. Additionally, the transcriptomes and proteomes of two representative strains, one from each of the two main groups, have been characterized to evaluate the impact of mutations on RNA and proteins levels. This study has revealed that type 2 strains show higher expression levels of CARDS toxin, a protein recently shown to be one of the major factors of inflammation. Thus, we propose that type 2 strains could be more toxigenic than type 1 strains of M. pneumoniae.
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| Overall design |
4 strains (6009, 2882, 5817, 5954) were analyzed, with two biological replicates
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| Contributor(s) |
Lluch Senar M, Cozzuto L |
| Citation missing |
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| Submission date |
Jul 28, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Luca Cozzuto |
| E-mail(s) |
luca.cozzuto@crg.eu
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| Organization name |
Centre for genomic regulation (CRG)
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| Department |
CRG core facilities
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| Lab |
Bioinformatics Unit
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| Street address |
Carrer Dr. Aiguader 88
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| City |
Barcelona |
| State/province |
Barcelona |
| ZIP/Postal code |
08005 |
| Country |
Spain |
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| Platforms (1) |
| GPL20750 |
Illumina HiSeq 2000 (Mycoplasma pneumoniae) |
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| Samples (8)
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| GSM1835181 |
Mycoplasma pneumoniae (strain 6009)_CRO6 |
| GSM1835182 |
Mycoplasma pneumoniae (strain 6009)_CRO8 |
| GSM1835183 |
Mycoplasma pneumoniae (strain 2882)_FIL6 |
| GSM1835184 |
Mycoplasma pneumoniae (strain 2882)_FIL8 |
| GSM1835185 |
Mycoplasma pneumoniae (strain 5817)_PAU6 |
| GSM1835186 |
Mycoplasma pneumoniae (strain 5817)_PAU8 |
| GSM1835187 |
Mycoplasma pneumoniae (strain 5954)_PER6 |
| GSM1835188 |
Mycoplasma pneumoniae (strain 5954)_PER8 |
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| Relations |
| BioProject |
PRJNA291356 |
| SRA |
SRP061742 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE71467_RAW.tar |
130.0 Kb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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