|
| Status |
Public on Jun 26, 2015 |
| Title |
caArray_krahe-00060: Expression profiling reveals fundamental biological differences in acute myeloid leukemia |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Acute myeloid leukemia (AML) is a heterogeneous group of diseases. Normal cytogenetics (CN) constitutes the single largest group, while trisomy 8 (+8) as a sole abnormality is the most frequent trisomy. How trisomy contributes to tumorigenesis is unknown. We used oligonucleotide-based DNA microarrays to study global gene expression in AML+8 patients with +8 as the sole chromosomal abnormality and AML-CN patients. CD34+ cells purified from normal bone marrow (BM) were also analyzed as a representative heterogeneous population of stem and progenitor cells. Expression patterns of AML patients were clearly distinct from those of CD34+ cells of normal individuals. We show that AML+8 blasts overexpress genes on chromosome 8, estimated at 32% on average, suggesting gene-dosage effects underlying AML+8. Systematic analysis by cellular function indicated up-regulation of genes involved in cell adhesion in both groups of AML compared with CD34+ blasts from normal individuals. Perhaps most interestingly, apoptosis-regulating genes were significantly down-regulated in AML+8 compared with AML-CN. We conclude that the clinical and cytogenetic heterogeneity of AML is due to fundamental biological differences.
**NOTE: Migrated from caArray 1.x, identifier='gov.nih.nci.ncicb.caarray:Experiment:1015897559579654:1'
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| |
|
| Overall design |
krahe-00060
Assay Type: Gene Expression
Provider: Affymetrix
Array Designs: Hu6800
Organism: Homo sapiens (ncbitax)
Tissue Sites: Bone marrow
Material Types: synthetic_DNA, synthetic_RNA, organism_part
Cell Types: Myeloid Cell
Disease States: Acute Myeloid Leukemia, Normal
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| |
|
| Citation(s) |
11158605 |
| Submission date |
Jun 25, 2015 |
| Last update date |
Jul 08, 2016 |
| Contact name |
Mervi Heiskanen |
| E-mail(s) |
mervi.heiskanen@nih.gov
|
| Phone |
2402765175
|
| Organization name |
NCI
|
| Department |
CBIIT
|
| Street address |
9609 Medical Center Drive, 1W378
|
| City |
Rockville |
| State/province |
MD |
| ZIP/Postal code |
20850 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL80 |
[Hu6800] Affymetrix Human Full Length HuGeneFL Array |
|
| Samples (31)
|
|
| Relations |
| BioProject |
PRJNA288156 |
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