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| Status |
Public on Jun 25, 2015 |
| Title |
caArray_hanas-00078: Distinctive molecular profiles of high-grade and low-grade gliomas based on oligonucleotide microarray analysis |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Astrocytomas are heterogeneous intracranial glial neoplasms ranging from the highly aggressive malignant glioblastoma multiforme (GBM) to the indolent, low-grade pilocytic astrocytoma. We have investigated whether DNA microarrays can identify gene expression differences between high-grade and low-grade glial tumors. We compared the transcriptional profile of 45 astrocytic tumors including 21 GBMs and 19 pilocytic astrocytomas using oligonucleotide-based microarrays. Of the approximately 6800 genes that were analyzed, a set of 360 genes provided a molecular signature that distinguished between GBMs and pilocytic astrocytomas. Many transcripts that were increased in GBM were not previously associated with gliomas and were found to encode proteins with properties that suggest their involvement in cell proliferation or cell migration. Microarray-based data for a subset of genes was validated using real-time quantitative reverse transcription-PCR. Immunohistochemical analysis also localized the protein products of specific genes of interest to the neoplastic cells of high-grade astrocytomas. Our study has identified a large number of novel genes with distinct expression patterns in high-grade and low-grade gliomas.
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| |
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| Overall design |
hanas-00078
Assay Type: Gene Expression
Provider: Affymetrix
Array Designs: Hu6800
Organism: Homo sapiens (ncbitax)
Tissue Sites: Brain
Material Types: synthetic_DNA, synthetic_RNA, organism_part
Disease States: Primary Glioma, Normal
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| |
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| Citation(s) |
11559565 |
| Submission date |
Jun 24, 2015 |
| Last update date |
Jul 08, 2016 |
| Contact name |
Mervi Heiskanen |
| E-mail(s) |
mervi.heiskanen@nih.gov
|
| Phone |
2402765175
|
| Organization name |
NCI
|
| Department |
CBIIT
|
| Street address |
9609 Medical Center Drive, 1W378
|
| City |
Rockville |
| State/province |
MD |
| ZIP/Postal code |
20850 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL80 |
[Hu6800] Affymetrix Human Full Length HuGeneFL Array |
|
| Samples (51)
|
|
| Relations |
| BioProject |
PRJNA287927 |
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