|
| Status |
Public on Jan 20, 2007 |
| Title |
Expression data from head and neck squamous cell carcinoma |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Forty-four paired (from the same patient) samples of head and neck squamous cell carcinoma (HNSCC) and normal tissue were studied with Affymetrix U95A chips. A stringent multi-test approach, combining 7 traditional and microarray-specific statistical tests, was used to analyze the resultant data. Candidate genes were assigned to tiers of significance based on the number of statistical tests that each gene satisfied. Representative genes (both up-regulated and down-regulated) from each of the 3 tiers would be quantified with RT-PCR on both microarray-tested and new samples of HNSCC.
The goal of this study is to identify reliable differentially-expressed genes on HNSCC and to testify our hypothesis whether or not a combinatorial approach (multi-tests) to analyzing microarray data can really identify differentially-expressed genes with fewer false-positives.
Keywords: disease state; tumor vs. normal
|
| |
|
| Overall design |
Paired HNSCC tumor and normal samples from 22 patients were evaluated for differential gene expression on Affymetrix U95A chips.
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| |
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| Contributor(s) |
Kuriakose MA, Chen WT, He ZM, Sikora AG, Zhang P, Zhang ZY, Qiu WL, Hsu DF, McMunn-Coffran C, Brown SM, Elongo EM, Delacure MD, Chen FA |
| Citation(s) |
15170515 |
| Submission date |
Jan 03, 2007 |
| Last update date |
Dec 13, 2018 |
| Contact name |
Fang-An Chen |
| E-mail(s) |
chenf01@nyu.edu
|
| Phone |
212-998-9564
|
| Fax |
212-995-4087
|
| Organization name |
New York Univ. College of Dentistry
|
| Department |
Oral and Maxillifacial surgery
|
| Street address |
345E 24th Street
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10010 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL8300 |
[HG_U95Av2] Affymetrix Human Genome U95 Version 2 Array |
|
| Samples (44)
|
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| Relations |
| BioProject |
PRJNA98861 |
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