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| Status |
Public on Jan 30, 2007 |
| Title |
Zymosan stimulation of MyD88-deficient bone marrow-derived macrophages |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Innate immune pattern recognition receptors play critical roles in pathogen detection and initiation of antimicrobial responses. We and others have previously demonstrated the importance of the beta-glucan receptor Dectin-1 in the recognition of pathogenic fungi by macrophages and dendritic cells, and have elucidated some of the mechanisms by which Dectin-1 signals to coordinate the antifungal response. While Dectin-1 signals alone are sufficient to trigger phagocytosis and Src-Syk-mediated induction of antimicrobial reactive oxygen species, collaboration with Toll-like receptor (TLR)2 signaling enhances NF-kB activation and regulates cytokine production. In this study we demonstrate that Dectin-1 signaling can also directly modulate gene expression via activation of nuclear transcription of activated T cells (NFAT) transcription factors. Dectin-1 ligation by zymosan particles or live Candida albicans yeast triggers NFAT activation in macrophages and dendritic cells. Dectin-1-triggered NFAT activation plays a role in the induction of Egr2 and Egr3 transcription factors, and cyclooxygenase 2 (Cox-2). Furthermore, we show that NFAT activation regulates IL-2, IL-10 and IL-12 p70 production by zymosan-stimulated dendritic cells. These data establish NFAT activation in myeloid cells as a novel mechanism of regulation of the innate antimicrobial response.
Keywords: stress response
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| Overall design |
Bone marrow-derived macrophages deficient in MyD88 were stimulated with zymosan, and total RNA was extracted 120 minutes after stimulation for comparison to macrophages grown under the same conditions, but not stimulated.
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| Contributor(s) |
Goodridge HS, Simmons RS, Underhill DM |
| Citation(s) |
17312158 |
| Submission date |
Nov 27, 2006 |
| Last update date |
Aug 02, 2019 |
| Contact name |
Bruz Marzolf |
| E-mail(s) |
bmarzolf@systemsbiology.org
|
| Phone |
206-732-1352
|
| Organization name |
Institute for Systems Biology
|
| Street address |
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98103 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA99601 |
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