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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Dec 14, 2006 |
| Title |
Gene Expression Profiling Reveals Unique Pathways Associated with Differential Severity of Lyme Arthritis |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The murine model of Lyme disease provides a unique opportunity to study the localized host response to similar stimulus, B. burgdorferi, in the joints of mice destined to develop severe arthritis (C3H) or mild disease (C57BL/6). Pathways associated with the response to infection and the development of Lyme arthritis were identified by global gene expression patterns using oligonucleotide microarrays. A robust induction of IFN responsive genes was observed in severely arthritic C3H mice at one week of infection, which was absent from mildly arthritic C57BL/6 mice. In contrast, infected C57BL/6 mice displayed a novel expression profile characterized by genes involved in epidermal differentiation and wound repair, which were decreased in the joints of C3H mice. These expression patterns were associated with disease state rather than inherent differences between C3H and C57BL/6 mice, as C57BL/6-IL10-/- mice infected with B. burgdorferi develop more severe arthritis that C57BL/6 mice and displayed an early gene expression profile similar to C3H mice. Gene expression profiles at two and four weeks post infection revealed a common response of all strains that was likely to be important for the host defense to B. burgdorferi and mediated by NF-kB-dependent signaling. The gene expression profiles identified in this study add to the current understanding of the host response to B. burgdorferi and identify two novel pathways that may be involved in regulating the severity of Lyme arthritis.
Keywords: disease state analysis, time course
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| Overall design |
Expression profiling of ankle tissues of C3H, C57BL/6, and C57BL/6-IL10-/- mice infected with B. burgdorfer (0, 1, 2, and 4 weeks post-infection)
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| Contributor(s) |
Crandall H, Dunn DM, Ma Y, Wooten RM, Zachary JF, Weis JH, Weis RB, Weis JJ |
| Citation(s) |
17114465 |
| Submission date |
Oct 17, 2006 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Janis Weis |
| E-mail(s) |
janis.weis@path.utah.edu
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| Phone |
801-581-8386
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| Fax |
801-581-4517
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| Organization name |
University of Utah
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| Department |
Pathology
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| Street address |
15 North Medical Drive East
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| City |
Salt Lake City |
| State/province |
UT |
| ZIP/Postal code |
84112-5650 |
| Country |
USA |
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| Platforms (2) |
| GPL339 |
[MOE430A] Affymetrix Mouse Expression 430A Array |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (12)
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| GSM140228 |
Expression data from ankle tissue of C3H mice at 0 week of infection (uninfected) |
| GSM140229 |
Expression data from ankle tissue of C3H mice at 1 week of infection |
| GSM140230 |
Expression data from ankle tissue of C3H mice at 2 week of infection |
| GSM140231 |
Expression data from ankle tissue of C3H mice at 4 week of infection |
| GSM140561 |
Expression data from ankle tissue of C57BL/6 mice at 0 week of infection (uninfected) |
| GSM140562 |
Expression data from ankle tissue of C57BL/6 mice at 1 week of infection |
| GSM140563 |
Expression data from ankle tissue of C57BL/6 mice at 2 week of infection |
| GSM140564 |
Expression data from ankle tissue of C57BL/6 mice at 4 week of infection |
| GSM140565 |
Expression data from ankle tissue of C57BL/6 IL10KO mice at 0 week of infection (uninfected) |
| GSM140566 |
Expression data from ankle tissue of C57BL/6 IL10KO mice at 1 week of infection |
| GSM140567 |
Expression data from ankle tissue of C57BL/6 IL10KO mice at 2 week of infection |
| GSM140568 |
Expression data from ankle tissue of C57BL/6 IL10KO mice at 4 week of infection |
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| Relations |
| BioProject |
PRJNA97733 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE6055_RAW.tar |
59.9 Mb |
(http)(custom) |
TAR (of CEL) |
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