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| Status |
Public on Jul 11, 2014 |
| Title |
Monocytic cell differentiation from band-stage neutrophils under inflammatory conditions |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
During inflammation, neutrophils are rapidly mobilized from the bone marrow storage pool into peripheral blood to subsequently enter lesional sites where most of them rapidly undergo apoptosis. Monocytes constitute a second wave of inflammatory immigrates giving rise to long-lived macrophages and dendritic cell subsets. According to descriptive immunophenotypic and cell culture studies, neutrophils may directly “transdifferentiate” into monocytes/macrophages. We here provide mechanistic data in human supporting the existence of this cellular pathway. Global transcriptional profiling of neutrophil-dervived monocytic cells revealed close resemblance of gene expression with monocytes and a clear separation of these cells from neutrophils.
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| Overall design |
G-CSF mobilized neutrophils from peripheral blood were isolated and in vitro stimulated with GM-CSF, TNFa, and Il-1b for 5 days to generate monocytic cells. RNA were isolated from these cells and freshly isolated blood neutrophils and monocytes.
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| |
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| Contributor(s) |
Köffel R, Wagner K, Strobl H |
| Citation(s) |
25214442 |
| Submission date |
Jun 30, 2014 |
| Last update date |
Mar 15, 2019 |
| Contact name |
Karin Wagner |
| E-mail(s) |
kar.wagner@medunigraz.at
|
| Organization name |
Medical University Graz
|
| Department |
Molecular Biology
|
| Street address |
Stiftingtalstrasse 24
|
| City |
Graz |
| State/province |
Styria |
| ZIP/Postal code |
8010 |
| Country |
Austria |
| |
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| Platforms (1) |
| GPL16686 |
[HuGene-2_0-st] Affymetrix Human Gene 2.0 ST Array [transcript (gene) version] |
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| Samples (14)
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| Relations |
| BioProject |
PRJNA253926 |
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