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| Status |
Public on Nov 12, 2014 |
| Title |
Transcriptome Analysis of the Human Corneal Endothelium |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Defining the normal and age-dependent HCEnC transcriptome will further refine our understanding of the functional roles that the endothelium plays in the cornea and will provide a basis upon which to compare transcriptomes of normal and dystrophic endothelium for the subsequent development of gene-targeted therapies.
We used microarrays to comprehensively characterize human corneal endothelial cell (HCEnC) gene expression, age-dependent differential gene expression and to identify expressed genes mapped to chromosomal loci associated with the corneal endothelial dystrophies PPCD1, FECD4 and XECD
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| Overall design |
Eleven corneas from six pediatric (4, 6, 10,11, 17 and 18 years old) and five adult (53, 56, 57, 64 and 70 years old) donors, were obtained from various eye banks . Descemet and corneal endothelium were removed from donor eye bank corneas as intact sheets utilizing a technique that we have adapted for ex vivo donor tissue. Total RNA was isolated from the corneal endothelium using TriReagent and subsequently purified with the RNeasy Clean-Up Kit. The integrity of the isolated RNA was analyzed using the Agilent 2100 Electrophoresis Bioanalyzer System and found to be of sufficient quantity (approximately 1ug) and quality (RNA integrity number in the range of 8.3 to 9.0) for analysis using the Affymetrix 1.1ST gene chip arrays.
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| Contributor(s) |
Frausto RF, Wang C, Aldave AJ |
| Citation(s) |
25377225 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 EY022082 |
Identification and Characterization of the Genetic Basis of PPCD |
UNIVERSITY OF CALIFORNIA LOS ANGELES |
ALDAVE |
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| Submission date |
Jun 09, 2014 |
| Last update date |
Nov 08, 2016 |
| Contact name |
Anthony J. Aldave |
| E-mail(s) |
Aldave@jsei.ucla.edu
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| Organization name |
Stein Eye Institute, UCLA
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| Department |
Ophthalmology
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| Lab |
Cornea Genetics Laboratory
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| Street address |
200 Stein Plaza DSERC 3-143
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| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90095 |
| Country |
USA |
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| Platforms (1) |
| GPL11532 |
[HuGene-1_1-st] Affymetrix Human Gene 1.1 ST Array [transcript (gene) version] |
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| Samples (11)
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| GSM1406380 |
HCEnC, adult, adult 4 |
| GSM1406381 |
HCEnC, pediatric, adolescent 5 |
| GSM1406382 |
HCEnC, adult, adult 6 |
| GSM1406383 |
HCEnC, pediatric, adolescent 7 |
| GSM1406384 |
HCEnC, pediatric, preschooler 8 |
| GSM1406385 |
HCEnC, pediatric, preschooler 9 |
| GSM1406386 |
HCEnC, adult, adult 10 |
| GSM1406387 |
HCEnC, adult, adult 11 |
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| Relations |
| BioProject |
PRJNA251984 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE58315_RAW.tar |
45.7 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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