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GEO help: Mouse over screen elements for information. |
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| Status |
Public on May 10, 2016 |
| Title |
Expression data from 1499 cells, a mouse ADM/PanIN1-derived pancreatic ductal cell line, and the mouse pancreatic ductal adenocarcinoma (PDAC) AH375 cell line. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
In a Kras-driven mouse model of multistage pancreatic cancer progression, decreased p-ERK levels correlate with tumor initiation. In cells derived from this model, transformed cells with low p-ERK levels express markers of pluripotency and demonstrate phenotypes of tumor initiating cells, such as formation of free-floating tumor spheres. Here, a comparison of gene expression from the 1499 cell line, which are premalignant cells isolated from mouse ADM (acinar-to-ductal metaplasia) and PanIN1 (pancreatic intraepithelial lesions), and the AH375 cell line, established from mouse PDAC, was performed.
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| Overall design |
To establish the 1499 cell line, cells were isolated from premalignant pancreatic lesions (ADM and PanIN1) in a 9-week-old Pdx1-Cre;LSL-KRasG12D mouse. To establish the AH375 cell line, cells were isolated from PDAC in a 52-week-old Pdx1-Cre;LSL-KRasG12D mouse. These pancreatic ductal cells were propagated on surfaces coated with laminin and in a specific pancreatic medium. Total mRNA extracts were prepared in triplicates for both cell lines and sent to the Génome Québec Innovation Centre for RNA processing and data acquisition.
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| Contributor(s) |
Deschênes-Simard X, Bardeesy N, Ferbeyre G |
| Citation(s) |
30614183, 31831715 |
| Submission date |
May 12, 2014 |
| Last update date |
Jan 14, 2020 |
| Contact name |
Xavier Deschênes-Simard |
| E-mail(s) |
xavier.deschenes-simard@mail.mcgill.ca
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| Organization name |
McGill University
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| Department |
Medicine
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| Street address |
3655 Sir William Osler
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| City |
Montréal |
| State/province |
Québec |
| ZIP/Postal code |
H3G 1Y6 |
| Country |
Canada |
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| Platforms (1) |
| GPL16570 |
[MoGene-2_0-st] Affymetrix Mouse Gene 2.0 ST Array [transcript (gene) version] |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA246713 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE57566_RAW.tar |
51.8 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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