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Status |
Public on Aug 20, 2008 |
Title |
Gene expression analysis of T-ALL cell lines treated with gamma-secretase inhibitor |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array
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Summary |
Gain-of-function mutations in NOTCH1 are common in T-cell lymphoblastic leukemias making this receptor a promising target for drugs such as gamma-secretase inhibitors (GSI), which block a proteolytic cleavage required for NOTCH1 activation. However, the enthusiasm for these therapies has been tempered by tumor resistance and the paucity of information on the oncogenic programs regulated by oncogenic NOTCH1. Analysis of gene expression in GSI-responsive and GSI-resistant cell lines treated with Compound E identifies differential resopnses to GSI. Keywords: Drug response
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Overall design |
Samples for microarray analysis were prepared and hybridized in Affymetrix Human U133 Plus 2.0 arrays according to the manufacturer’s instructions and as previously described. RNA was extracted from duplicate cultures of GSI-sensitive (ALL-SIL, CUTLL1, DND41, HPB-ALL, KOPTK1) and GSI-resistant (CCRF-CEM, MOLT3, P12 ICHIKAWA, PF382 and RPMI8402) T-ALL cell lines treated for 24 h with vehicle (DMSO) or 500 nM CompE. Interarray intensity differences were normalized with Dchip.
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Contributor(s) |
Palomero T, Sulis M, Cortina M, Real PJ, Barnes K, Ciofani M, Caparros E, Buteau J, Brown K, Perkins SL, Bhagat G, Mishra A, Basso G, Parsons R, Zúñiga-Pflücker J, Dominguez M, Ferrando AA |
Citation(s) |
17873882 |
Submission date |
Sep 01, 2006 |
Last update date |
Mar 25, 2019 |
Contact name |
Adolfo A Ferrando |
E-mail(s) |
af2196@columbia.edu
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Phone |
212-851-4611
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Organization name |
Columbia University
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Department |
Institute for Cancer Genetics
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Street address |
1130 St Nicholas Ave Irving Cancer Research Center 5-505A
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City |
New York |
State/province |
NY |
ZIP/Postal code |
10032 |
Country |
USA |
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Platforms (1) |
GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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Samples (40)
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Relations |
BioProject |
PRJNA97007 |
Supplementary file |
Size |
Download |
File type/resource |
GSE5716_RAW.tar |
117.0 Mb |
(http)(custom) |
TAR (of CEL) |
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